LS-C378148

antibody from LSBio

Targeting: SHISA5 hShisa5, SCOTIN

ELISA (Recommended by provider)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C378148

Provider

LSBio

Product name

SHISA5 / SCOTIN Antibody LS-C378148

Antibody type

Polyclonal

Description

Protein G purified

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Short term: -20°C; Long term: -80°C; Avoid freeze-thaw cycles.

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunoprecipitating SHISA5 in HepG2 whole cell lysate Lane 1: Rabbit control IgG (1µg) instead of SHISA5 Antibody in HepG2 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: SHISA5 Antibody (6µg) + HepG2 whole cell lysate (500µg) Lane 3: HepG2 whole cell lysate (10µg)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating SHISA5 in HepG2 whole cell lysate Lane 1: Rabbit control IgG (1µg) instead of SHISA5 Antibody in HepG2 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: SHISA5 Antibody (6µg) + HepG2 whole cell lysate (500µg) Lane 3: HepG2 whole cell lysate (10µg)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating SHISA5 in HepG2 whole cell lysate Lane 1: Rabbit control IgG (1µg) instead of SHISA5 Antibody in HepG2 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: SHISA5 Antibody (6µg) + HepG2 whole cell lysate (500µg) Lane 3: HepG2 whole cell lysate (10µg)

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human adrenal gland tissue using antibody at dilution of 1:100.