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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
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- Product number
- AF5557 - Provider product page
- Provider
- R&D Systems
- Product name
- Human PREB Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human PREB in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q9HCU5- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references The ER-Golgi intermediate compartment is a key membrane source for the LC3 lipidation step of autophagosome biogenesis.
Sec12 binds to Sec16 at transitional ER sites.
Ge L, Melville D, Zhang M, Schekman R
eLife 2013 Aug 6;2:e00947
eLife 2013 Aug 6;2:e00947
Sec12 binds to Sec16 at transitional ER sites.
Montegna EA, Bhave M, Liu Y, Bhattacharyya D, Glick BS
PloS one 2012;7(2):e31156
PloS one 2012;7(2):e31156
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Human PREB by Western Blot. Western blot shows lysates of human tonsil tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human PREB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5557) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for PREB at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
