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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
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- Product number
- PA5-20437 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD248 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control is human colon tissue lysate. PA5-20437 can be used with blocking peptide PEP-0554. The PA5-20437 immunogen is located within amino acids 40-90 of TEM-1. Predicted molecular ~ 81kD. In Western blot applications, this antibody has been observed to detect a band at: 90 kD (post-modifications: 27 O-linked glycosylations). Predicted species reactivity based on immunogen sequence: Mouse (100%); Rat: (93%).
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot Validation in Human Colon Tissue. Loading: 15 µg of lysates per lane. Antibodies: CD248 Polyclonal Antibody (Product # PA5-20437) (A, 0.5 µg/mL; B, 1 µg/mL), 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot Validation in Human Tissue. Loading: 15 µg of lysates per lane. Antibodies: CD248 Polyclonal Antibody (Product # PA5-20437) (0.5 µg/mL), 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of 4% paraformaldehyde-fixed Human Colon Tissue labeling TEM-1 with CD248 Polyclonal Antibody (Product # PA5-20437) at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1:500 dilution (red).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Human Colon tissue using CD248 Polyclonal Antibody (Product # PA5-20437) at 2.5 µg/mL. Tissue was fixed with formaldehyde and blocked with 0.1 serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
