MA5-50430

antibody from Invitrogen Antibodies

Targeting: PFKFB4

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-50430

Provider

Invitrogen Antibodies

Product name

PFKFB4 Recombinant Rabbit Monoclonal Antibody (HL2204)

Antibody type

Monoclonal

Antigen

Recombinant protein fragment

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

HL2204

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of PFKFB4 using PFKFB4 Recombinant Monoclonal Antibody (Product # MA5-50430) in U87-MG (30 μg) and Molt-4 (30 μg) whole cell extracts at a dilution of 1:1,000. Extracts were separated by 10% SDS-PAGE. An HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of PFKFB4 using PFKFB4 Recombinant Monoclonal Antibody (Product # MA5-50430) in TM4 (30 μg) and Hepa1-6 (30 μg) whole cell extracts at a dilution of 1:1,000. Extracts were separated by 10% SDS-PAGE. An HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of PFKFB4 using PFKFB4 Recombinant Monoclonal Antibody (Product # MA5-50430) in Rat2 whole cell extract (30 μg) at a dilution of 1:1,000. Extracts were separated by 10% SDS PAGE. An HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of PFKFB4 using PFKFB4 Recombinant Monoclonal Antibody (Product # MA5-50430) in Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) at a dilution of 1:5,000. Extracts were separated by 10% SDS PAGE. An HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of PFKFB4 using PFKFB4 Recombinant Monoclonal Antibody (Product # MA5-50430) in U87-MG (30 μg) and Molt-4 (30 μg) whole cell extracts at a dilution of 1:1,000. Extracts were separated by 10% SDS-PAGE. An HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of PFKFB4 using PFKFB4 Recombinant Monoclonal Antibody (Product # MA5-50430) in Paraffin embedded mouse spleen tissue at a dilution of 1:100. For Antigen retrieval citrate buffer, pH 6.0, as used for 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of PFKFB4 using PFKFB4 Recombinant Monoclonal Antibody (Product # MA5-50430) in Paraffin embedded rat spleen tissue at a dilution of 1:100. For Antigen retrieval citrate buffer, pH 6.0, as used for 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of PFKFB4 using PFKFB4 Recombinant Monoclonal Antibody (Product # MA5-50430) in Paraffin embedded rat spleen tissue at a dilution of 1:100. For Antigen retrieval citrate buffer, pH 6.0, as used for 15 min.