PA5-28648

antibody from Invitrogen Antibodies

Targeting: PFKFB4

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-28648

Provider

Invitrogen Antibodies

Product name

PFKFB4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: Raji, mouse liver, rat liver.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PFKFB4 using 50 µg mouse liver lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a PFKFB4 polyclonal antibody (Product # PA5-28648) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PFKFB4 using 50 µg rat liver lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a PFKFB4 polyclonal antibody (Product # PA5-28648) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PFKFB4 was performed by separating 50 µg of rat tissue extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a PFKFB4 Polyclonal Antibody (Product # PA5-28648) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using PFKFB4 Polyclonal Antibody (Product # PA5-28648). Sample (30 µg whole cell lysate). A: Raji. 10% SDS PAGE. PFKFB4 Polyclonal Antibody (Product # PA5-28648) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PFKFB4 Polyclonal Antibody detects PFKFB4 protein at cytoplasm by immunofluorescent analysis. Sample: NT2D1 cells were fixed in ice-cold MeOH for 5 min. Green: PFKFB4 protein stained by PFKFB4 Polyclonal Antibody (Product # PA5-28648) diluted at 1:500. Blue: Hoechst 33342 staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of PFKFB4 was performed in paraffin-embedded mouse testis tissue using PFKFB4 Polyclonal Antibody (Product # PA5-28648) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using PFKFB4 (Product # PA5-28648) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PFKFB4 Polyclonal Antibody detects PFKFB4 protein at cytoplasm on mouse spleen by immunohistochemical analysis. Sample: Paraffin-embedded mouse spleen. PFKFB4 Polyclonal Antibody (Product # PA5-28648) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.