PA5-28648

antibody from Invitrogen Antibodies

Targeting: PFKFB4

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-28648

Provider

Invitrogen Antibodies

Product name

PFKFB4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Recommended positive controls: Raji, mouse liver, rat liver. Predicted reactivity: Mouse (97%), Rat (97%), Xenopus laevis (85%), Chicken (86%), Bovine (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 μL

Concentration

0.73 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PFKFB4 Polyclonal Antibody detects PFKFB4 protein at cytoplasm by immunofluorescent analysis. Sample: NT2D1 cells were fixed in ice-cold MeOH for 5 min. Green: PFKFB4 protein stained by PFKFB4 Polyclonal Antibody (Product # PA5-28648) diluted at 1:500. Blue: Hoechst 33342 staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PFKFB4 Polyclonal Antibody detects PFKFB4 protein at cytoplasm by immunofluorescent analysis. Sample: NT2D1 cells were fixed in ice-cold MeOH for 5 min. Green: PFKFB4 protein stained by PFKFB4 Polyclonal Antibody (Product # PA5-28648) diluted at 1:500. Blue: Hoechst 33342 staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PFKFB4 Polyclonal Antibody detects PFKFB4 protein at cytoplasm on mouse spleen by immunohistochemical analysis. Sample: Paraffin-embedded mouse spleen. PFKFB4 Polyclonal Antibody (Product # PA5-28648) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using PFKFB4 (Product # PA5-28648) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of PFKFB4 was performed in paraffin-embedded mouse testis tissue using PFKFB4 Polyclonal Antibody (Product # PA5-28648) at a dilution of 1:500.