PA1-32415

antibody from Invitrogen Antibodies

Targeting: SCARB2 CD36L2, HLGP85, LIMP-2, LIMPII, SR-BII

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-32415

Provider

Invitrogen Antibodies

Product name

SR-BI/SR-BII Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

The PA1-32415 immunogen is: Synthetic peptide from the extracellular domain (residues 230-380) of Scavenger Receptor-BI/BII was expressed as two tandem copies in bacteria using the pET system. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

Conc. Not Determined

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of SR-BI/SR-BII was performed by loading 80 µg of mouse liver tissue lysates. Proteins were transferred to a membrane and probed with a SR-BI/SR-BII Polyclonal Antibody (Product # PA1-32415). Lane 1: wild-type mice , Lane 2: SR-BI deficient mice.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Scavenger Receptor SR-BI + SR-BII in Hela cells using a Scavenger Receptor SR-BI + SR-BII polyclonal antibody (Product # PA1-32415) and detected with a Dylight 488 labeled secondary antibody (Green). Nuclei were visualized using Hoechst 33258 counterstain (Blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of SR-BI/SR-BII in HeLa cells using SR-BI/SR-BII Polyclonal Antibody (Product # PA1-32415) (Green). Red : Tubulin. Blue: DAPI.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of SR-BI/SR-BII in human liver tissue using SR-BI/SR-BII Polyclonal Antibody (Product # PA1-32415) at a dilution of 5 µg/mL.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometry analysis of PEX19 was performed in NIH-3T3 cells using PEX19 Monoclonal Antibody (GT554) (Product # MA5-17266) (Purple) at a dilution of 1:400. M1 is defined by unstained cells.