Antibody data
- Antibody Data
- Antigen structure
- References [16]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [3]
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Validation data
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- Product number
- NB400-129 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB400-129, RRID:AB_2301298
- Product name
- Rabbit Polyclonal LIMPII/SR-B2 Antibody
- Antibody type
- Polyclonal
- Description
- Unpurified.
- Reactivity
- Human, Mouse, Rat, Canine, Simian
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Early trypsin activation develops independently of autophagy in caerulein-induced pancreatitis in mice.
β2-adrenergic Agonists Rescue Lysosome Acidification and Function in PSEN1 Deficiency by Reversing Defective ER-to-lysosome Delivery of ClC-7.
Intra-host emergence of an enterovirus A71 variant with enhanced PSGL1 usage and neurovirulence.
Granulovacuolar degeneration bodies are neuron-selective lysosomal structures induced by intracellular tau pathology.
Age-related endolysosome dysfunction in the rat urothelium.
Glutamine-dependent lysosome homeostatic changes induced by starvation and lysosome inhibition.
Mutation in VPS33A affects metabolism of glycosaminoglycans: a new type of mucopolysaccharidosis with severe systemic symptoms.
Evaluation of AAV-mediated Gene Therapy for Central Nervous System Disease in Canine Mucopolysaccharidosis VII.
Lysosome associated membrane proteins maintain pancreatic acinar cell homeostasis: LAMP-2 deficient mice develop pancreatitis.
Tight junctional localization of claudin-16 is regulated by syntaxin 8 in renal tubular epithelial cells.
Action myoclonus-renal failure syndrome: diagnostic applications of activity-based probes and lipid analysis.
High-density lipoprotein endocytosis in endothelial cells.
Abnormal Processing of Autophagosomes in Transformed B Lymphocytes from SCARB2-Deficient Subjects.
Whole body correction of mucopolysaccharidosis IIIA by intracerebrospinal fluid gene therapy.
Expression of the transmembrane lysosomal protein SCARB2/Limp-2 in renin secretory granules controls renin release.
A nonsense mutation in the LIMP-2 gene associated with progressive myoclonic epilepsy and nephrotic syndrome.
Malla SR, Krueger B, Wartmann T, Sendler M, Mahajan UM, Weiss FU, Thiel FG, De Boni C, Gorelick FS, Halangk W, Aghdassi AA, Reinheckel T, Gukovskaya AS, Lerch MM, Mayerle J
Cellular and molecular life sciences : CMLS 2020 May;77(9):1811-1825
Cellular and molecular life sciences : CMLS 2020 May;77(9):1811-1825
β2-adrenergic Agonists Rescue Lysosome Acidification and Function in PSEN1 Deficiency by Reversing Defective ER-to-lysosome Delivery of ClC-7.
Lee JH, Wolfe DM, Darji S, McBrayer MK, Colacurcio DJ, Kumar A, Stavrides P, Mohan PS, Nixon RA
Journal of molecular biology 2020 Apr 3;432(8):2633-2650
Journal of molecular biology 2020 Apr 3;432(8):2633-2650
Intra-host emergence of an enterovirus A71 variant with enhanced PSGL1 usage and neurovirulence.
Sun L, Tijsma A, Mirabelli C, Baggen J, Wahedi M, Franco D, De Palma A, Leyssen P, Verbeken E, van Kuppeveld FJM, Neyts J, Thibaut HJ
Emerging microbes & infections 2019;8(1):1076-1085
Emerging microbes & infections 2019;8(1):1076-1085
Granulovacuolar degeneration bodies are neuron-selective lysosomal structures induced by intracellular tau pathology.
Wiersma VI, van Ziel AM, Vazquez-Sanchez S, Nölle A, Berenjeno-Correa E, Bonaterra-Pastra A, Clavaguera F, Tolnay M, Musters RJP, van Weering JRT, Verhage M, Hoozemans JJM, Scheper W
Acta neuropathologica 2019 Dec;138(6):943-970
Acta neuropathologica 2019 Dec;138(6):943-970
Age-related endolysosome dysfunction in the rat urothelium.
Truschel ST, Clayton DR, Beckel JM, Yabes JG, Yao Y, Wolf-Johnston A, Birder LA, Apodaca G
PloS one 2018;13(6):e0198817
PloS one 2018;13(6):e0198817
Glutamine-dependent lysosome homeostatic changes induced by starvation and lysosome inhibition.
Wilden AR, Molina JA, Feuerborn M, Boyle D, Lee SY
Biochimica et biophysica acta. Molecular cell research 2018 Sep;1865(9):1356-1367
Biochimica et biophysica acta. Molecular cell research 2018 Sep;1865(9):1356-1367
Mutation in VPS33A affects metabolism of glycosaminoglycans: a new type of mucopolysaccharidosis with severe systemic symptoms.
Kondo H, Maksimova N, Otomo T, Kato H, Imai A, Asano Y, Kobayashi K, Nojima S, Nakaya A, Hamada Y, Irahara K, Gurinova E, Sukhomyasova A, Nogovicina A, Savvina M, Yoshimori T, Ozono K, Sakai N
Human molecular genetics 2017 Jan 1;26(1):173-183
Human molecular genetics 2017 Jan 1;26(1):173-183
Evaluation of AAV-mediated Gene Therapy for Central Nervous System Disease in Canine Mucopolysaccharidosis VII.
Gurda BL, De Guilhem De Lataillade A, Bell P, Zhu Y, Yu H, Wang P, Bagel J, Vite CH, Sikora T, Hinderer C, Calcedo R, Yox AD, Steet RA, Ruane T, O'Donnell P, Gao G, Wilson JM, Casal M, Ponder KP, Haskins ME
Molecular therapy : the journal of the American Society of Gene Therapy 2016 Feb;24(2):206-216
Molecular therapy : the journal of the American Society of Gene Therapy 2016 Feb;24(2):206-216
Lysosome associated membrane proteins maintain pancreatic acinar cell homeostasis: LAMP-2 deficient mice develop pancreatitis.
Mareninova OA, Sendler M, Malla SR, Yakubov I, French SW, Tokhtaeva E, Vagin O, Oorschot V, Lüllmann-Rauch R, Blanz J, Dawson D, Klumperman J, Lerch MM, Mayerle J, Gukovsky I, Gukovskaya AS
Cellular and molecular gastroenterology and hepatology 2015 Nov 1;1(6):678-694
Cellular and molecular gastroenterology and hepatology 2015 Nov 1;1(6):678-694
Tight junctional localization of claudin-16 is regulated by syntaxin 8 in renal tubular epithelial cells.
Ikari A, Tonegawa C, Sanada A, Kimura T, Sakai H, Hayashi H, Hasegawa H, Yamaguchi M, Yamazaki Y, Endo S, Matsunaga T, Sugatani J
The Journal of biological chemistry 2014 May 9;289(19):13112-23
The Journal of biological chemistry 2014 May 9;289(19):13112-23
Action myoclonus-renal failure syndrome: diagnostic applications of activity-based probes and lipid analysis.
Gaspar P, Kallemeijn WW, Strijland A, Scheij S, Van Eijk M, Aten J, Overkleeft HS, Balreira A, Zunke F, Schwake M, Sá Miranda C, Aerts JM
Journal of lipid research 2014 Jan;55(1):138-45
Journal of lipid research 2014 Jan;55(1):138-45
High-density lipoprotein endocytosis in endothelial cells.
Fruhwürth S, Pavelka M, Bittman R, Kovacs WJ, Walter KM, Röhrl C, Stangl H
World journal of biological chemistry 2013 Nov 26;4(4):131-40
World journal of biological chemistry 2013 Nov 26;4(4):131-40
Abnormal Processing of Autophagosomes in Transformed B Lymphocytes from SCARB2-Deficient Subjects.
Gleich K, Desmond MJ, Lee D, Berkovic SF, Dibbens LM, Katerelos M, Bayly MA, Fraser SA, Martinello P, Vears DF, Mount P, Power DA
BioResearch open access 2013 Feb;2(1):40-6
BioResearch open access 2013 Feb;2(1):40-6
Whole body correction of mucopolysaccharidosis IIIA by intracerebrospinal fluid gene therapy.
Haurigot V, Marcó S, Ribera A, Garcia M, Ruzo A, Villacampa P, Ayuso E, Añor S, Andaluz A, Pineda M, García-Fructuoso G, Molas M, Maggioni L, Muñoz S, Motas S, Ruberte J, Mingozzi F, Pumarola M, Bosch F
The Journal of clinical investigation 2013 Aug 1;123(8):3254-3271
The Journal of clinical investigation 2013 Aug 1;123(8):3254-3271
Expression of the transmembrane lysosomal protein SCARB2/Limp-2 in renin secretory granules controls renin release.
Lee D, Desmond MJ, Fraser SA, Katerelos M, Gleich K, Berkovic SF, Power DA
Nephron. Experimental nephrology 2012;122(3-4):103-13
Nephron. Experimental nephrology 2012;122(3-4):103-13
A nonsense mutation in the LIMP-2 gene associated with progressive myoclonic epilepsy and nephrotic syndrome.
Balreira A, Gaspar P, Caiola D, Chaves J, Beirão I, Lima JL, Azevedo JE, Miranda MC
Human molecular genetics 2008 Jul 15;17(14):2238-43
Human molecular genetics 2008 Jul 15;17(14):2238-43
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: LIMPII/SR-B2 Antibody [NB400-129] - CHAPS-solublized SVG-A (human astrocyte cells) and B103 (rat neuroblastoma cells) cell lyates, at the indicated concentrations, were probed with a 1:25 dilution of antibody. Image from verified customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: LIMPII/SR-B2 Antibody [NB400-129] - Detection of LIMP II (~85 kDa) in Lane 1: Rat cardiac fibroblasts (RCF), 3T3 mouse fibroblasts (2), canine lung lysate (3), RCF treated with angiotensin II (4), and H2O2 (5), HEPG2 human liver lysate (6).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: LIMPII/SR-B2 Antibody [NB400-129] - Staining of LIMP II in rat infarctzone tissue with a DAB detection system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: LIMPII/SR-B2 Antibody [NB400-129] - Lysotracker Red accumulates in endolysosomes.Immunofluorescence of young (A) and aged (C) urothelium incubated with Lysotracker Red (red) and co-stained with antibodies to LIMP2 (green structures) and phalloidin (blue), which labels the cortical actin cytoskeleton. Large, Lysotracker Red-positive endolysosomes are marked with white arrows. Underlying intermediate (IC) and basal cells (BC) show non-specific cytoplasmic Lysotracker Red staining that did not appear in umbrella cells (UC). Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0198817), licensed under a CC-BY licence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: LIMPII/SR-B2 Antibody [NB400-129] - Comparison of LIMP2-positive organelles in young (A) and aged (C) rat urothelium. LIMP2-positive structures (green) shown in umbrella cells (delineated by dashed yellow line). Rhodamine phalloidin (red), which labels the cortical actin cytoskeleton, was used to show cell borders. Umbrella cells show an apparent, age-related increase in LIMP2 staining (white arrows). Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0198817), licensed under a CC-BY licence.