702770

antibody from Invitrogen Antibodies

Targeting: SCARB2 CD36L2, HLGP85, LIMP-2, LIMPII, SR-BII

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

702770

Provider

Invitrogen Antibodies

Product name

LIMP2 Recombinant Rabbit Monoclonal Antibody (12H5L1)

Antibody type

Monoclonal

Antigen

Other

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Antibody clone number

12H5L1

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Knockdown of SCARB2 was achieved by transfecting SH-SY5Y cells with SCARB2 specific validated siRNA (Silencer® select Product # s2651). Western blot analysis (Fig a) was performed using membrane enriched extracts from SCARB2 knockdown cells (Lane 3), non-specific scrambled siRNA transfected cells (Lane 2) and untransfected cells (Lane 1). The blots were probed with Anti-LIMP2 Recombinant Rabbit Monoclonal Antibody (Product # 702770, 1:500 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). Densitometric analysis of this Western blot is shown in histogram (Fig b). Loss of signal upon siRNA mediated knock down confirms that antibody is specific to SCARB2.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on Membrane enriched extracts (30 µg lysate) of U-87 MG (Lane 1), IMR-32 (Lane 2), 3T3-L1 (Lane 3), HeLa (Lane 4), MCF-7 (Lane 5) and tissue extracts of Mouse Brain (Lane 6),Rat Kidney (Lane 7) and Mouse Liver (Lane 8). The blots were probed with Anti-LIMP2 Recombinant Rabbit Monoclonal Antibody (Product # 702770, 1:500 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 77 kDa band corresponding to SCARB2 was observed across the cell lines and tissues tested.