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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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Validation data
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- Product number
- 702770 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LIMP2 Recombinant Rabbit Monoclonal Antibody (12H5L1)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- This antibody is predicted to react with Monkey, Rabbit.
- Antibody clone number
- 12H5L1
- Concentration
- 0.5 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of SCARB2 was achieved by transfecting SH-SY5Y cells with SCARB2 specific validated siRNA (Silencer® select Product # s2651). Western blot analysis (Fig a) was performed using membrane enriched extracts from SCARB2 knockdown cells (Lane 3), non-specific scrambled siRNA transfected cells (Lane 2) and untransfected cells (Lane 1). The blots were probed with Anti-LIMP2 Recombinant Rabbit Monoclonal Antibody (Product # 702770, 1:500 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). Densitometric analysis of this Western blot is shown in histogram (Fig b). Loss of signal upon siRNA mediated knock down confirms that antibody is specific to SCARB2.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on Membrane enriched extracts (30 µg lysate) of U-87 MG (Lane 1), IMR-32 (Lane 2), 3T3-L1 (Lane 3), HeLa (Lane 4), MCF-7 (Lane 5) and tissue extracts of Mouse Brain (Lane 6),Rat Kidney (Lane 7) and Mouse Liver (Lane 8). The blots were probed with Anti-LIMP2 Recombinant Rabbit Monoclonal Antibody (Product # 702770, 1:500 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 77 kDa band corresponding to SCARB2 was observed across the cell lines and tissues tested.
