PA3-16805

antibody from Invitrogen Antibodies

Targeting: SCARB2 CD36L2, HLGP85, LIMP-2, LIMPII, SR-BII

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Blocking/Neutralizing (Recommended by provider)

Antibody Data

Product number

PA3-16805

Provider

Invitrogen Antibodies

Product name

SR-BI/SR-BII Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

Conc. Not Determined

Storage

-20°C, Avoid Freeze/Thaw Cycles

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of SR-BI/SR-BII in HeLa cells. Samples were incubated in SR-BI/SR-BII polyclonal antibody (Product # PA3-16805) followed by DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of SR-BI/SR-BII in formalin-fixed paraffin-embedded tissue section of normal human liver. Samples were incubated in SR-BI/SR-BII polyclonal antibody (Product # PA3-16805) using a dilution of 5 µg/mL. Specific and expected granular membrane-cytoplasmic staining was observed in the hepatocytes. [40X Magnification].

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

SR-BI/II staining in mouse subcutaneous tissue post-injury. Tissues were fixed in Zinc-Tris buffer overnight, blocked with 2.5% rabbit serum, and stained with Scavenger Receptor B1/2 Polyclonal Antibody (Product # PA3-16805) at 1:1000 dilution followed by a biotinylated-goat anti-rabbit IgG secondary antibody, avidin-biotin reagent and DAB substrate. Data courtesy of the Innovators Program

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of SR-BI/SR-BII in NIH-3T3 cells. Samples were incubated in SR-BI/SR-BII polyclonal antibody (Product # PA3-16805) using a dilution of 1:400 followed by a Alexa Fluor 488 secondary (shown in purple). M1 is defined by unstained cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of SR-BI/SR-BII in NIH-3T3 cells. Samples were incubated in SR-BI/SR-BII polyclonal antibody (Product # PA3-16805) using a dilution of 1:400 followed by a Alexa Fluor 488 secondary (shown in purple). M1 is defined by unstained cells.