Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- AF4344 - Provider product page
- Provider
- R&D Systems
- Product name
- Human PON2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects endogenous human PON2 in Western blots. In Western blots, this antibody shows no cross-reactivity with recombinant human PON1 or PON3.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q15165
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Paraoxonase 2 overexpression inhibits tumor development in a mouse model of ovarian cancer.
Devarajan A, Su F, Grijalva V, Yalamanchi M, Yalamanchi A, Gao F, Trost H, Nwokedi J, Farias-Eisner G, Farias-Eisner R, Fogelman AM, Reddy ST
Cell death & disease 2018 Mar 12;9(3):392
Cell death & disease 2018 Mar 12;9(3):392
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human PON2 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human PON2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4344) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PON2 at approximately 42-45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.