HPA016858

antibody from Atlas Antibodies

Targeting: ESYT1 FAM62A, KIAA0747, MBC2

Western blot (Enhanced validation)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

HPA016858

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA016858, RRID:AB_1848404

Product name

Anti-ESYT1

Antibody type

Polyclonal

Description

Polyclonal Antibody against Human ESYT1, Gene description: extended synaptotagmin-like protein 1, Alternative Gene Names: FAM62A, KIAA0747, MBC2, Validated applications: WB, IHC, Uniprot ID: Q9BSJ8, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 µl

Concentration

0.1 mg/ml

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Handling

The antibody solution should be gently mixed before use.

References

Submitted references

Modulation of GPR133 (ADGRD1) signaling by its intracellular interaction partner extended synaptotagmin 1

Stephan G, Haddock S, Wang S, Erdjument-Bromage H, Liu W, Ravn-Boess N, Frenster J, Bready D, Cai J, Ronnen R, Sabio-Ortiz J, Fenyo D, Neubert T, Placantonakis D
Cell Reports 2024;43(5):114229

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Extended-Synaptotagmin 1 Enhances Liver Cancer Progression Mediated by the Unconventional Secretion of Cytosolic Proteins

Yamada K, Hannya Y, Oikawa T, Yoshida A, Katagiri K, Yoshida S, Koizumi R, Tago N, Shimoyama Y, Kawamura A, Mochimaru Y, Eto K, Yoshida K
Molecules 2023;28(10):4033

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PERK recruits E-Syt1 at ER–mitochondria contacts for mitochondrial lipid transport and respiration

Sassano M, van Vliet A, Vervoort E, Van Eygen S, Van den Haute C, Pavie B, Roels J, Swinnen J, Spinazzi M, Moens L, Casteels K, Meyts I, Pinton P, Marchi S, Rochin L, Giordano F, Felipe-Abrio B, Agostinis P
Journal of Cell Biology 2023;222(3)

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Dynamic interactome of the MHC I peptide loading complex in human dendritic cells

Barends M, Koller N, Schölz C, Durán V, Bošnjak B, Becker J, Döring M, Blees H, Förster R, Kalinke U, Tampé R
Proceedings of the National Academy of Sciences 2023;120(25)

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Stephan G, Erdjument-Bromage H, Liu W, Frenster J, Ravn-Boess N, Bready D, Cai J, Fenyo D, Neubert T, Placantonakis D
2023

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Extended-synaptotagmin 1 engages in unconventional protein secretion mediated via SEC22B+vesicle pathway in liver cancer

Yamada K, Motohashi S, Oikawa T, Tago N, Koizumi R, Ono M, Tachibana T, Yoshida A, Yoshida S, Shimoda M, Oka M, Yoneda Y, Yoshida K
Proceedings of the National Academy of Sciences 2022;119(36)

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E-syt1 Re-arranges STIM1 Clusters to Stabilize Ring-shaped ER-PM Contact Sites and Accelerate Ca2+ Store Replenishment

Kang F, Zhou M, Huang X, Fan J, Wei L, Boulanger J, Liu Z, Salamero J, Liu Y, Chen L
Scientific Reports 2019;9(1)

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Triggered Ca 2+ influx is required for extended synaptotagmin 1‐induced ER ‐plasma membrane tethering

Idevall‐Hagren O, Lü A, Xie B, De Camilli P
The EMBO Journal 2015;34(17):2291-2305

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Three-dimensional architecture of extended synaptotagmin-mediated endoplasmic reticulum–plasma membrane contact sites

Fernández-Busnadiego R, Saheki Y, De Camilli P
Proceedings of the National Academy of Sciences 2015;112(16)

Western blot

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ESYT1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Sample type

Human

Protocol

Protocol