A301-631A

antibody from Invitrogen Antibodies

Targeting: TRERF1 BCAR2, dJ139D8.5, HSA277276, RAPA, TReP-132

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

A301-631A

Provider

Invitrogen Antibodies

Product name

TReP-132/TRERF1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human TReP-132/TRERF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TReP-132/TRERF1 antibody A301-631A (lot A301-631A-2) used for IP at 3 µg per reaction. TReP-132/TRERF1 was also immunoprecipitated by rabbit anti-TReP-132/TRERF1 antibody A301-630A. For blotting immunoprecipitated TReP-132/TRERF1, A301-631A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human TReP-132/TRERF1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TReP-132/TRERF1 antibody A301-631A (lot A301-631A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human TReP-132/TRERF1 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-TReP-132/TRERF1 (Cat. No. A301-631A Lot2) used at a dilution of 1:5,000 (0.2µg/ml). Detection: DAB.