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Western blot
Immunocytochemistry
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [2]
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- Product number
- MA5-27592 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NPAS4 Monoclonal Antibody (N408/79)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- A 1:100 dilution of MA5-27592 was sufficient for detection of NPAS4 in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 90kDa.approximately. This antibody was formerly sold as clone S408-79.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- N408/79
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references Nuclear receptor binding factor 2 (NRBF2) is required for learning and memory.
Ouyang X, Ahmad I, Johnson MS, Redmann M, Craver J, Wani WY, Benavides GA, Chacko B, Li P, Young M, Jegga AG, Darley-Usmar V, Zhang J
Laboratory investigation; a journal of technical methods and pathology 2020 Sep;100(9):1238-1251
Laboratory investigation; a journal of technical methods and pathology 2020 Sep;100(9):1238-1251
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of NPAS4 in human neuroblastoma cell line (SK-N-BE). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with NPAS4 monoclonal antibody (Product # MA5-27592) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Mouse 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:100, 1:1000 (60 min at RT) and 1:5000 (5 min at RT). Images are shown as follows: (A) DAPI (blue) nuclear stain B) Phalloidin Texas Red F-Actin stain C) NPAS4 Antibody D) Merged image. Magnification: 60x.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of NPAS4 in human neuroblastoma cell line (SK-N-BE). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with NPAS4 monoclonal antibody (Product # MA5-27592) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Mouse 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:100, 1:1000 (60 min at RT) and 1:5000 (5 min at RT). Images are shown as follows: (A) DAPI (blue) nuclear stain B) Phalloidin Texas Red F-Actin stain C) NPAS4 Antibody D) Merged image. Magnification: 60x.
