PA5-21834

antibody from Invitrogen Antibodies

Targeting: ASB4 ASB-4

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-21834

Provider

Invitrogen Antibodies

Product name

ASB4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: A431, Mouse brain. Predicted reactivity: Mouse (94%), Rat (94%), Bovine (96%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.74 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ASB4 using 30µg of A431 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with an ASB4 polyclonal antibody (Product # PA5-21834) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of ASB4 was performed by separating 30 µg of whole cell extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a ASB4 Polyclonal Antibody (Product # PA5-21834) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using ASB4 Polyclonal Antibody (Product # PA5-21834). Sample (50 µg of whole cell lysate). Lane A: Mouse brain. 10% SDS PAGE. ASB4 Polyclonal Antibody (Product # PA5-21834) diluted at 1:1,000.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using ASB4 (Product # PA5-21834) antibody at 1:100 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.