MA5-32006

antibody from Invitrogen Antibodies

Targeting: IFNAR1 IFNAR, IFRC

Western blot (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-32006

Provider

Invitrogen Antibodies

Product name

IFNAR1 Recombinant Rabbit Monoclonal Antibody (SR45-08)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Antibody clone number

SR45-08

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

5-Fluorouracil efficacy requires anti-tumor immunity triggered by cancer-cell-intrinsic STING.

Tian J, Zhang D, Kurbatov V, Wang Q, Wang Y, Fang D, Wu L, Bosenberg M, Muzumdar MD, Khan S, Lu Q, Yan Q, Lu J
The EMBO journal 2021 Apr 1;40(7):e106065

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometric analysis of IFNAR1 in Jurkat cells using a IFNAR1 Monoclonal Antibody (Product # MA5-32006) at a dilution of 1:50, as seen in blue compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

5 Figure Efficient 5-FU-induced tumor inhibition depends on IFN-sensing by bone-marrow-derived cells A Western blot for Ctrl and Ifnar1-KO MC38 cells, analyzed with Ifnar1 and GAPDH antibodies. B Ctrl or Ifnar1-KO MC38 cells were treated with PBS or recombinant Ifnbeta for 4 h. The RNA expression levels of indicated ISGs were analyzed using qRT-PCR. N = 3. C-E Mice were injected with control (Ctrl) or Ifnar1-KO MC38 cells, and treated with 5-FU or PBS. (C) Pictures of tumors and spleens from a representative experiment. (D) Tumor volumes were quantified at the indicated days after cancer cell injection. N = 5. (E) Tumor and spleen weights at the endpoint for (D), with each dot representing a mouse. Ctrl tumor data in (C-E) are the same as those in Fig 4B-D. F-I Schematics (F) of the experiment to test the function of Ifnar1 in bone-marrow (BM)-derived cells. WT C57BL/6 mice were transplanted with either Ifnar1 +/+ or Ifnar1 -/- BM cells. Recipient mice were allowed to recover followed by the injection of WT MC38 cells, before treatment with 5-FU or PBS. (G) Pictures of tumors and spleens from a representative experiment. Image panels were cropped from the same picture. (H) Tumor volumes were quantified at the indicated days after cancer cell injection. N = 4 to N = 5, as shown in (G). (I) Tumor and spleen weights at the endpoint for (H), with each dot representing a mouse. Data information: For all panels, error bars stand for SD, and center values represent mean. Two-tailed