Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AP08683PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP08683PU-N, RRID:AB_1975577
- Product name
- anti IFNAR1 pSer535/539
- Antibody type
- Polyclonal
- Antigen
- Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser535,539 of Human IFNAR1.
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Sheep
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
Submitted references Inducible priming phosphorylation promotes ligand-independent degradation of the IFNAR1 chain of type I interferon receptor.
Phosphorylation and specific ubiquitin acceptor sites are required for ubiquitination and degradation of the IFNAR1 subunit of type I interferon receptor.
Bhattacharya S, HuangFu WC, Liu J, Veeranki S, Baker DP, Koumenis C, Diehl JA, Fuchs SY
The Journal of biological chemistry 2010 Jan 22;285(4):2318-25
The Journal of biological chemistry 2010 Jan 22;285(4):2318-25
Phosphorylation and specific ubiquitin acceptor sites are required for ubiquitination and degradation of the IFNAR1 subunit of type I interferon receptor.
Kumar KG, Krolewski JJ, Fuchs SY
The Journal of biological chemistry 2004 Nov 5;279(45):46614-20
The Journal of biological chemistry 2004 Nov 5;279(45):46614-20
No comments: Submit comment
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Figure 1. Western blot of immunoprecipitates from HEK 293 cells transfected with: 1. Mock, 2. IFNAR1 WT, and 3. IFNAR1 S535A and S539A mutants showing specific immunolabeling of the ~110k to ~130k IFNAR1 WT. The immunolabeling is absent in IFNAR1 Ser535 and Ser539 mutants (Control). The immunolabeling is blocked by the phosphopeptide (Phos) used as the antigen but not by the corresponding dephosphopeptide (Dephos).