AP08683PU-N

antibody from Acris Antibodies GmbH

Targeting: IFNAR1 IFNAR, IFRC

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

AP08683PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AP08683PU-N, RRID:AB_1975577

Product name

anti IFNAR1 pSer535/539

Antibody type

Polyclonal

Antigen

Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser535,539 of Human IFNAR1.

Reactivity

Human, Mouse, Rat, Bovine, Canine, Sheep

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

References

Submitted references

Inducible priming phosphorylation promotes ligand-independent degradation of the IFNAR1 chain of type I interferon receptor.

Bhattacharya S, HuangFu WC, Liu J, Veeranki S, Baker DP, Koumenis C, Diehl JA, Fuchs SY
The Journal of biological chemistry 2010 Jan 22;285(4):2318-25

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Phosphorylation and specific ubiquitin acceptor sites are required for ubiquitination and degradation of the IFNAR1 subunit of type I interferon receptor.

Kumar KG, Krolewski JJ, Fuchs SY
The Journal of biological chemistry 2004 Nov 5;279(45):46614-20

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Figure 1. Western blot of immunoprecipitates from HEK 293 cells transfected with: 1. Mock, 2. IFNAR1 WT, and 3. IFNAR1 S535A and S539A mutants showing specific immunolabeling of the ~110k to ~130k IFNAR1 WT. The immunolabeling is absent in IFNAR1 Ser535 and Ser539 mutants (Control). The immunolabeling is blocked by the phosphopeptide (Phos) used as the antigen but not by the corresponding dephosphopeptide (Dephos).