V8778

antibody from NSJ Bioreagents

Targeting: UBR2 bA49A4.1, C6orf133, dJ392M17.3, KIAA0349

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Protein array (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

V8778

Provider

NSJ Bioreagents

Product name

UBR2 Antibody / E3 ubiquitin-protein ligase UBR2

Antibody type

Monoclonal

Description

This highly specific UBR2 antibody is suitable for use in Immunoprecipitation/Flow cytometry/Immunofluorescence/Western blot/Immunohistochemistry applications with human samples.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

PCRP-UBR2-1D12

Vial size

20 ug (with BSA and sodium azide), 100 ug (with BSA and sodium azide), 100 ug (without BSA or sodium azide), 7 ml IHC only format (if applicable)

Concentration

0.2 mg/ml, 1 mg/ml

Storage

Aliquot the UBR2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of PFA-fixed human HeLa cells using UBR2 antibody (green, clone PCRP-UBR2-1D12) and phalloidin (red).

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human thyroid tissue with UBR2 antibody (clone PCRP-UBR2-1D12) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

FACS staining of PFA-fixed human HeLa cells with UBR2 antibody (blue, clone PCRP-UBR2-1D12) and isotype control (red).

Protein array

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using UBR2 antibody (clone PCRP-UBR2-1D12). These results demonstrate the foremost specificity of the PCRP-UBR2-1D12 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Other assay

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

SDS-PAGE analysis of purified, BSA-free UBR2 antibody (PCRP-UBR2-1D12) as confirmation of integrity and purity.