H00121441-M05

antibody from Novus Biologicals

Targeting: NEDD1 GCP-WD, TUBGCP7

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00121441-M05

Provider

Novus Biologicals

Proper citation

Novus Cat#H00121441-M05, RRID:AB_2149199

Product name

Mouse Monoclonal NEDD1 Antibody

Antibody type

Monoclonal

Description

IgG purified. NEDD1 - neural precursor cell expressed, developmentally down-regulated 1

Reactivity

Human, Mouse, Rat, Bovine, Porcine, Sheep

Host

Mouse

Isotype

IgG

Vial size

0.1 mg

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

References

Submitted references

Error-prone meiotic division and subfertility in mice with oocyte-conditional knockdown of pericentrin.

Baumann C, Wang X, Yang L, Viveiros MM
Journal of cell science 2017 Apr 1;130(7):1251-1262

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Augmin shapes the anaphase spindle for efficient cytokinetic furrow ingression and abscission.

Uehara R, Kamasaki T, Hiruma S, Poser I, Yoda K, Yajima J, Gerlich DW, Goshima G
Molecular biology of the cell 2016 Mar 1;27(5):812-27

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The dynamics of microtubule minus ends in the human mitotic spindle.

Lecland N, Lüders J
Nature cell biology 2014 Aug;16(8):770-8

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Impact of vitrification on the meiotic spindle and components of the microtubule-organizing center in mouse mature oocytes.

Tamura AN, Huang TT, Marikawa Y
Biology of reproduction 2013 Nov;89(5):112

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SPICE--a previously uncharacterized protein required for centriole duplication and mitotic chromosome congression.

Archinti M, Lacasa C, Teixidó-Travesa N, Lüders J
Journal of cell science 2010 Sep 15;123(Pt 18):3039-46

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Functional central spindle assembly requires de novo microtubule generation in the interchromosomal region during anaphase.

Uehara R, Goshima G
The Journal of cell biology 2010 Oct 18;191(2):259-67

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Axon extension occurs independently of centrosomal microtubule nucleation.

Stiess M, Maghelli N, Kapitein LC, Gomis-Rüth S, Wilsch-Bräuninger M, Hoogenraad CC, Tolić-Nørrelykke IM, Bradke F
Science (New York, N.Y.) 2010 Feb 5;327(5966):704-7

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Plk1-dependent recruitment of gamma-tubulin complexes to mitotic centrosomes involves multiple PCM components.

Haren L, Stearns T, Lüders J
PloS one 2009 Jun 19;4(6):e5976

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FAM29A, a target of Plk1 regulation, controls the partitioning of NEDD1 between the mitotic spindle and the centrosomes.

Zhu H, Fang K, Fang G
Journal of cell science 2009 Aug 1;122(Pt 15):2750-9

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FAM29A promotes microtubule amplification via recruitment of the NEDD1-gamma-tubulin complex to the mitotic spindle.

Zhu H, Coppinger JA, Jang CY, Yates JR 3rd, Fang G
The Journal of cell biology 2008 Dec 1;183(5):835-48

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: NEDD1 Antibody (7D10) [H00121441-M05] - Western Blot analysis of NEDD1 expression in Hela NE ( Cat # L013V3 ).

ELISA

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

ELISA: NEDD1 Antibody (7D10) [H00121441-M05] - Detection limit for recombinant GST tagged NEDD1 is approximately 0.03ng/ml as a capture antibody.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: NEDD1 Antibody (7D10) [H00121441-M05] - Analysis of monoclonal antibody to NEDD1 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 0.75 ug/ml