LS-C676142

antibody from LSBio

Targeting: NEDD1 GCP-WD, TUBGCP7

Western blot (Enhanced validation)

ELISA (Recommended by provider)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

LS-C676142

Provider

LSBio

Product name

NEDD1 Antibody LS-C676142

Antibody type

Polyclonal

Description

Immunoaffinity purified

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot All Lanes:NEDD1 antibody at 2.66 ug/ml Lane 1: Mouse brain tissue Lane 2: Jurkat whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 72,63,73 kDa Observed band size: 72,35 kDa

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot All lanes: NEDD1 antibody at 2.66µg/ml Lane 1: Mouse brain tissue Lane 2: Jurkat whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 72, 63, 73 kDa Observed band size: 72 kDa

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunoprecipitating NEDD1 in HeLa whole cell lysate Lane 1: Rabbit monoclonal IgG(1ug)instead of product in HeLa whole cell lysate.For western blotting, a HRP-conjugated light chain specific antibody was used as the Secondary antibody (1/50000) Lane 2: product(4ug)+ HeLa whole cell lysate(500ug) Lane 3: HeLa whole cell lysate (20ug)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating NEDD1 in HeLa whole cell lysate Lane 1: Rabbit monoclonal IgG(1ug)instead of product in HeLa whole cell lysate.For western blotting, a HRP-conjugated light chain specific antibody was used as the Secondary antibody (1/50000) Lane 2: product(4ug)+ HeLa whole cell lysate(500ug) Lane 3: HeLa whole cell lysate (20ug)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating NEDD1 in HeLa whole cell lysate Lane 1: Rabbit monoclonal IgG(1ug)instead of product in HeLa whole cell lysate.For western blotting, a HRP-conjugated light chain specific antibody was used as the Secondary antibody (1/50000) Lane 2: product(4ug)+ HeLa whole cell lysate(500ug) Lane 3: HeLa whole cell lysate (20ug)