PA5-27653

antibody from Invitrogen Antibodies

Targeting: LYPLA2 APT-2

Western blot (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-27653

Provider

Invitrogen Antibodies

Product name

LYPLA2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: 293T, A431, HeLaS3, HepG2, Molt-4, Raji. Predicted reactivity: Mouse (99%), Rat (99%), Chicken (88%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Temporal Profiling Establishes a Dynamic S-Palmitoylation Cycle.

Won SJ, Martin BR
ACS chemical biology 2018 Jun 15;13(6):1560-1568

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 5. Steady-state S -palmitoylation is unaffected in APT1 -/- /APT2 -/- mouse brains. (a) FP-TAMRA labeling of active serine hydrolases in whole mouse brain homogenates confirms selective deletion of APT1 and APT2 with no observable compensatory changes across other hydrolases. Knockout was also confirmed by western blot. Labeling is shown with 2 uM FP-TAMRA. (b) Hydroxylamine-switch analysis of S -palmitoylation in normal and knockout samples does not reveal major changes in S-acylation profiles. Detection with 1 uM IAM-TAMRA. (c) Volcano plot analysis of acyl-RAC enrichment and multiplexed TMT mass spectrometry analysis of APT1 -/- /APT2 -/- whole mouse brain homogenates (N=3). Red lines represent p < 0.05 from two-tailed Student's t-test with unequal variance (horizontal) and 2-fold change (vertical).