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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Other assay [1]
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- Product number
- PA5-27653 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LYPLA2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLaS3, HepG2, Molt-4, Raji. Predicted reactivity: Mouse (99%), Rat (99%), Chicken (88%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Temporal Profiling Establishes a Dynamic S-Palmitoylation Cycle.
Won SJ, Martin BR
ACS chemical biology 2018 Jun 15;13(6):1560-1568
ACS chemical biology 2018 Jun 15;13(6):1560-1568
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of lysophospholipase II using 30 µg of A) HepG2 and B) Raji lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a lysophospholipase II polyclonal antibody (Product # PA5-27653) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using LYPLA2 Polyclonal Antibody (Product # PA5-27653). Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with LYPLA2 Polyclonal Antibody (Product # PA5-27653) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), HT-1080 (Lane 2), SNU-5 (Lane 3), Raji (Lane 4), Ramos (Lane 5), Neuro-2a (Lane 6) and tissue extract of Mouse Liver (Lane 7). The blot was probed with Anti-LYPLA2 Polyclonal Antibody (Product # PA5-27653, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 25 kDa band corresponding to LYPLA2 was observed across the cell lines and tissue tested.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5. Steady-state S -palmitoylation is unaffected in APT1 -/- /APT2 -/- mouse brains. (a) FP-TAMRA labeling of active serine hydrolases in whole mouse brain homogenates confirms selective deletion of APT1 and APT2 with no observable compensatory changes across other hydrolases. Knockout was also confirmed by western blot. Labeling is shown with 2 uM FP-TAMRA. (b) Hydroxylamine-switch analysis of S -palmitoylation in normal and knockout samples does not reveal major changes in S-acylation profiles. Detection with 1 uM IAM-TAMRA. (c) Volcano plot analysis of acyl-RAC enrichment and multiplexed TMT mass spectrometry analysis of APT1 -/- /APT2 -/- whole mouse brain homogenates (N=3). Red lines represent p < 0.05 from two-tailed Student's t-test with unequal variance (horizontal) and 2-fold change (vertical).
