PA5-21733

antibody from Invitrogen Antibodies

Targeting: TST RDS

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-21733

Provider

Invitrogen Antibodies

Product name

TST Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: HepG2, mouse liver, rat liver.

Concentration

0.39 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Rhodanese using 50 µg of mouse liver lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a Rhodanese polyclonal antibody (Product # PA5-21733) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Rhodanese using 50 µg rat liver lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a Rhodanese polyclonal antibody (Product # PA5-21733) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Rhodanese using 30 µg of HepG2 lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a Rhodanese polyclonal antibody (Product # PA5-21733) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using TST Polyclonal Antibody (Product # PA5-21733). Various tissue extracts (50 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with TST Polyclonal Antibody (Product # PA5-21733) diluted at 1:3,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using TST Polyclonal Antibody (Product # PA5-21733). Human tissue extract (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with TST Polyclonal Antibody (Product # PA5-21733) diluted at 1:3,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using TST (Product # PA5-21733) antibody at 1:250 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

TST Polyclonal Antibody detects TST protein at mitochondria on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. TST Polyclonal Antibody (Product # PA5-21733) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.