Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [5]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-30196 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TST Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HepG2, mouse liver, rat liver. Predicted reactivity: Mouse (89%), Rat (90%), Rhesus Monkey (96%), Bovine (90%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.71 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TST using 50 µg of mouse liver lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a TST polyclonal antibody (Product # PA5-30196) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TST using 50 µg rat liver lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a TST polyclonal antibody (Product # PA5-30196) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TST using 30 µg of HepG2 lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a TST polyclonal antibody (Product # PA5-30196) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using TST Polyclonal Antibody (Product # PA5-30196). Various tissue extracts (50 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with TST Polyclonal Antibody (Product # PA5-30196) diluted at 1:3,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using TST Polyclonal Antibody (Product # PA5-30196). Human tissue extract (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with TST Polyclonal Antibody (Product # PA5-30196) diluted at 1:3,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TST Polyclonal Antibody detects TST protein at cytosol on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. TST Polyclonal Antibody (Product # PA5-30196) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.