PA5-87925

antibody from Invitrogen Antibodies

Targeting: CTSF CATSF, CLN13

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-87925

Provider

Invitrogen Antibodies

Product name

Cathepsin F Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: LAPPEWDWRS KGAVTKVKDQ GMCGSCWAFS VTGNVEGQWF LNQGTLLSLS EQELLDCDKM DKACMGGLPS NAYSAIKNLG GLETEDDYSY QGHMQSCNFS AEKAKVYIND SVELSQNEQK LAAWLAKRGP ISVAINAFGM QFYRHGISRP LRPLCSPWLI DHAVLLVGYG NRSDVPFWAI KNSWGTDWGE KGYYYLHRGS GACGVNTMAS SAVVD; Positive Samples: U-251MG, SKOV3; Cellular Location: Lysosome

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

2.44 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Cathepsin F in extracts of various cell lines. Samples were incubated with Cathepsin F Polyclonal antibody (Product # PA5-87925) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Cathepsin F in paraffin-embedded human breast cancer using. Samples were incubated with Cathepsin F Polyclonal antibody (Product # PA5-87925) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Cathepsin F in paraffin-embedded human breast cancer using. Samples were incubated with Cathepsin F Polyclonal antibody (Product # PA5-87925) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.