Antibody data
- Antibody Data
- Antigen structure
- References [26]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 14681-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#14681-1-AP, RRID:AB_2060119
- Product name
- ASNS antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated ASNS antibody (Cat. #14681-1-AP) is a rabbit polyclonal antibody that shows reactivity with human and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Enhanced Antitumor Effect in Liver Cancer by Amino Acid Depletion-Induced Oxidative Stress.
Asparagine Synthetase-Mediated l-Asparagine Metabolism Disorder Promotes the Perineural Invasion of Oral Squamous Cell Carcinoma.
Canagliflozin Improves Liver Function in Rats by Upregulating Asparagine Synthetase.
Adaptation of the heart to Frataxin depletion: Evidence that integrated stress response can predominate over mTORC1 activation.
Asparagine couples mitochondrial respiration to ATF4 activity and tumor growth.
JMJD2B-induced amino acid alterations enhance the survival of colorectal cancer cells under glucose-deprivation via autophagy.
Glutamine depletion regulates Slug to promote EMT and metastasis in pancreatic cancer.
Glutamine and asparagine activate mTORC1 independently of Rag GTPases.
p53-mediated control of aspartate-asparagine homeostasis dictates LKB1 activity and modulates cell survival.
ZBTB1 Regulates Asparagine Synthesis and Leukemia Cell Response to L-Asparaginase.
Suppression of asparagine synthetase enhances the antitumor potency of ART and artemalogue SOMCL-14-221 in non-small cell lung cancer.
Amino acids signatures of distance-related surgical margins of oral squamous cell carcinoma.
Asparagine Deprivation Causes a Reversible Inhibition of Human Cytomegalovirus Acute Virus Replication.
Liver-Type Glutaminase GLS2 Is a Druggable Metabolic Node in Luminal-Subtype Breast Cancer.
The landscape of cancer cell line metabolism.
Asparagine Is a Critical Limiting Metabolite for Vaccinia Virus Protein Synthesis during Glutamine Deprivation.
Promoter demethylation of the asparagine synthetase gene is required for ATF4-dependent adaptation to asparagine depletion.
Autophagic reliance promotes metabolic reprogramming in oncogenic KRAS-driven tumorigenesis.
Arginine starvation kills tumor cells through aspartate exhaustion and mitochondrial dysfunction.
Arginine Deprivation Inhibits the Warburg Effect and Upregulates Glutamine Anaplerosis and Serine Biosynthesis in ASS1-Deficient Cancers.
Loss of asparagine synthetase suppresses the growth of human lung cancer cells by arresting cell cycle at G0/G1 phase.
Knockdown of asparagine synthetase by RNAi suppresses cell growth in human melanoma cells and epidermoid carcinoma cells.
Transcriptome Analysis of Piperlongumine-Treated Human Pancreatic Cancer Cells Reveals Involvement of Oxidative Stress and Endoplasmic Reticulum Stress Pathways.
Asparagine promotes cancer cell proliferation through use as an amino acid exchange factor.
Asparagine plays a critical role in regulating cellular adaptation to glutamine depletion.
Down-regulation of asparagine synthetase induces cell cycle arrest and inhibits cell proliferation of breast cancer.
Okuda K, Umemura A, Kataoka S, Yano K, Takahashi A, Okishio S, Taketani H, Seko Y, Nishikawa T, Yamaguchi K, Moriguchi M, Nakagawa H, Liu Y, Mitsumoto Y, Kanbara Y, Shima T, Okanoue T, Itoh Y
Frontiers in oncology 2021;11:758549
Frontiers in oncology 2021;11:758549
Asparagine Synthetase-Mediated l-Asparagine Metabolism Disorder Promotes the Perineural Invasion of Oral Squamous Cell Carcinoma.
Fu Y, Ding L, Yang X, Ding Z, Huang X, Zhang L, Chen S, Hu Q, Ni Y
Frontiers in oncology 2021;11:637226
Frontiers in oncology 2021;11:637226
Canagliflozin Improves Liver Function in Rats by Upregulating Asparagine Synthetase.
Wang S, Ding Y, Dong R, Wang H, Yin L, Meng S
Pharmacology 2021;106(11-12):606-615
Pharmacology 2021;106(11-12):606-615
Adaptation of the heart to Frataxin depletion: Evidence that integrated stress response can predominate over mTORC1 activation.
Vásquez-Trincado C, Patel M, Sivaramakrishnan A, Bekeová C, Anderson-Pullinger L, Wang N, Tang HY, Seifert EL
Human molecular genetics 2021 Sep 22;
Human molecular genetics 2021 Sep 22;
Asparagine couples mitochondrial respiration to ATF4 activity and tumor growth.
Krall AS, Mullen PJ, Surjono F, Momcilovic M, Schmid EW, Halbrook CJ, Thambundit A, Mittelman SD, Lyssiotis CA, Shackelford DB, Knott SRV, Christofk HR
Cell metabolism 2021 May 4;33(5):1013-1026.e6
Cell metabolism 2021 May 4;33(5):1013-1026.e6
JMJD2B-induced amino acid alterations enhance the survival of colorectal cancer cells under glucose-deprivation via autophagy.
Tan J, Wang HL, Yang J, Liu QQ, Li CM, Wang YQ, Fu LN, Gao QY, Chen YX, Fang JY
Theranostics 2020;10(13):5763-5777
Theranostics 2020;10(13):5763-5777
Glutamine depletion regulates Slug to promote EMT and metastasis in pancreatic cancer.
Recouvreux MV, Moldenhauer MR, Galenkamp KMO, Jung M, James B, Zhang Y, Lowy A, Bagchi A, Commisso C
The Journal of experimental medicine 2020 Sep 7;217(9)
The Journal of experimental medicine 2020 Sep 7;217(9)
Glutamine and asparagine activate mTORC1 independently of Rag GTPases.
Meng D, Yang Q, Wang H, Melick CH, Navlani R, Frank AR, Jewell JL
The Journal of biological chemistry 2020 Mar 6;295(10):2890-2899
The Journal of biological chemistry 2020 Mar 6;295(10):2890-2899
p53-mediated control of aspartate-asparagine homeostasis dictates LKB1 activity and modulates cell survival.
Deng L, Yao P, Li L, Ji F, Zhao S, Xu C, Lan X, Jiang P
Nature communications 2020 Apr 9;11(1):1755
Nature communications 2020 Apr 9;11(1):1755
ZBTB1 Regulates Asparagine Synthesis and Leukemia Cell Response to L-Asparaginase.
Williams RT, Guarecuco R, Gates LA, Barrows D, Passarelli MC, Carey B, Baudrier L, Jeewajee S, La K, Prizer B, Malik S, Garcia-Bermudez J, Zhu XG, Cantor J, Molina H, Carroll T, Roeder RG, Abdel-Wahab O, Allis CD, Birsoy K
Cell metabolism 2020 Apr 7;31(4):852-861.e6
Cell metabolism 2020 Apr 7;31(4):852-861.e6
Suppression of asparagine synthetase enhances the antitumor potency of ART and artemalogue SOMCL-14-221 in non-small cell lung cancer.
Xiao R, Ding C, Zhu H, Liu X, Gao J, Liu Q, Lu D, Zhang N, Zhang A, Zhou H
Cancer letters 2020 Apr 10;475:22-33
Cancer letters 2020 Apr 10;475:22-33
Amino acids signatures of distance-related surgical margins of oral squamous cell carcinoma.
Yang XH, Zhang XX, Jing Y, Ding L, Fu Y, Wang S, Hu SQ, Zhang L, Huang XF, Ni YH, Hu QG
EBioMedicine 2019 Oct;48:81-91
EBioMedicine 2019 Oct;48:81-91
Asparagine Deprivation Causes a Reversible Inhibition of Human Cytomegalovirus Acute Virus Replication.
Lee CH, Griffiths S, Digard P, Pham N, Auer M, Haas J, Grey F
mBio 2019 Oct 8;10(5)
mBio 2019 Oct 8;10(5)
Liver-Type Glutaminase GLS2 Is a Druggable Metabolic Node in Luminal-Subtype Breast Cancer.
Lukey MJ, Cluntun AA, Katt WP, Lin MJ, Druso JE, Ramachandran S, Erickson JW, Le HH, Wang ZE, Blank B, Greene KS, Cerione RA
Cell reports 2019 Oct 1;29(1):76-88.e7
Cell reports 2019 Oct 1;29(1):76-88.e7
The landscape of cancer cell line metabolism.
Li H, Ning S, Ghandi M, Kryukov GV, Gopal S, Deik A, Souza A, Pierce K, Keskula P, Hernandez D, Ann J, Shkoza D, Apfel V, Zou Y, Vazquez F, Barretina J, Pagliarini RA, Galli GG, Root DE, Hahn WC, Tsherniak A, Giannakis M, Schreiber SL, Clish CB, Garraway LA, Sellers WR
Nature medicine 2019 May;25(5):850-860
Nature medicine 2019 May;25(5):850-860
Asparagine Is a Critical Limiting Metabolite for Vaccinia Virus Protein Synthesis during Glutamine Deprivation.
Pant A, Cao S, Yang Z
Journal of virology 2019 Jul 1;93(13)
Journal of virology 2019 Jul 1;93(13)
Promoter demethylation of the asparagine synthetase gene is required for ATF4-dependent adaptation to asparagine depletion.
Jiang J, Srivastava S, Seim G, Pavlova NN, King B, Zou L, Zhang C, Zhong M, Feng H, Kapur R, Wek RC, Fan J, Zhang J
The Journal of biological chemistry 2019 Dec 6;294(49):18674-18684
The Journal of biological chemistry 2019 Dec 6;294(49):18674-18684
Autophagic reliance promotes metabolic reprogramming in oncogenic KRAS-driven tumorigenesis.
Lin HH, Chung Y, Cheng CT, Ouyang C, Fu Y, Kuo CY, Chi KK, Sadeghi M, Chu P, Kung HJ, Li CF, Limesand KH, Ann DK
Autophagy 2018;14(9):1481-1498
Autophagy 2018;14(9):1481-1498
Arginine starvation kills tumor cells through aspartate exhaustion and mitochondrial dysfunction.
Cheng CT, Qi Y, Wang YC, Chi KK, Chung Y, Ouyang C, Chen YR, Oh ME, Sheng X, Tang Y, Liu YR, Lin HH, Kuo CY, Schones D, Vidal CM, Chu JC, Wang HJ, Chen YH, Miller KM, Chu P, Yen Y, Jiang L, Kung HJ, Ann DK
Communications biology 2018;1:178
Communications biology 2018;1:178
Arginine Deprivation Inhibits the Warburg Effect and Upregulates Glutamine Anaplerosis and Serine Biosynthesis in ASS1-Deficient Cancers.
Kremer JC, Prudner BC, Lange SES, Bean GR, Schultze MB, Brashears CB, Radyk MD, Redlich N, Tzeng SC, Kami K, Shelton L, Li A, Morgan Z, Bomalaski JS, Tsukamoto T, McConathy J, Michel LS, Held JM, Van Tine BA
Cell reports 2017 Jan 24;18(4):991-1004
Cell reports 2017 Jan 24;18(4):991-1004
Loss of asparagine synthetase suppresses the growth of human lung cancer cells by arresting cell cycle at G0/G1 phase.
Xu Y, Lv F, Zhu X, Wu Y, Shen X
Cancer gene therapy 2016 Sep;23(9):287-94
Cancer gene therapy 2016 Sep;23(9):287-94
Knockdown of asparagine synthetase by RNAi suppresses cell growth in human melanoma cells and epidermoid carcinoma cells.
Li H, Zhou F, Du W, Dou J, Xu Y, Gao W, Chen G, Zuo X, Sun L, Zhang X, Yang S
Biotechnology and applied biochemistry 2016 May;63(3):328-33
Biotechnology and applied biochemistry 2016 May;63(3):328-33
Transcriptome Analysis of Piperlongumine-Treated Human Pancreatic Cancer Cells Reveals Involvement of Oxidative Stress and Endoplasmic Reticulum Stress Pathways.
Dhillon H, Mamidi S, McClean P, Reindl KM
Journal of medicinal food 2016 Jun;19(6):578-85
Journal of medicinal food 2016 Jun;19(6):578-85
Asparagine promotes cancer cell proliferation through use as an amino acid exchange factor.
Krall AS, Xu S, Graeber TG, Braas D, Christofk HR
Nature communications 2016 Apr 29;7:11457
Nature communications 2016 Apr 29;7:11457
Asparagine plays a critical role in regulating cellular adaptation to glutamine depletion.
Zhang J, Fan J, Venneti S, Cross JR, Takagi T, Bhinder B, Djaballah H, Kanai M, Cheng EH, Judkins AR, Pawel B, Baggs J, Cherry S, Rabinowitz JD, Thompson CB
Molecular cell 2014 Oct 23;56(2):205-218
Molecular cell 2014 Oct 23;56(2):205-218
Down-regulation of asparagine synthetase induces cell cycle arrest and inhibits cell proliferation of breast cancer.
Yang H, He X, Zheng Y, Feng W, Xia X, Yu X, Lin Z
Chemical biology & drug design 2014 Nov;84(5):578-84
Chemical biology & drug design 2014 Nov;84(5):578-84
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The ASNS antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human ASNS. This antibody recognizes human,mouse,rat antigen. The ASNS antibody has been validated for the following applications: ELISA, WB analysis.