PA5-56113

antibody from Invitrogen Antibodies

Targeting: ASNS

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-56113

Provider

Invitrogen Antibodies

Product name

Asparagine Synthetase Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: QHFEFEYQTK VDGEIILHLY DKGGIEQTIC MLDGVFAFVL LDTANKKVFL GRDTYGVRPL FKAMTEDGFL AVCSEAKGLV TLKHSATPFL KVEPFLPGHY EVLDLKPNGK VASVEMVKYH HCRDVP Highest antigen sequence identity to the following orthologs: Mouse - 94%, Rat - 93%.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.18 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Functional screening identifies aryl hydrocarbon receptor as suppressor of lung cancer metastasis.

Nothdurft S, Thumser-Henner C, Breitenbücher F, Okimoto RA, Dorsch M, Opitz CA, Sadik A, Esser C, Hölzel M, Asthana S, Forster J, Beisser D, Kalmbach S, Grüner BM, Bivona TG, Schramm A, Schuler M
Oncogenesis 2020 Nov 19;9(11):102

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Asparagine Synthetase using Asparagine Synthetase Polyclonal Antibody (Product # PA5-56113) (A) shows similar pattern to an independent Asparagine Synthetase Polyclonal Antibody (B).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 4 AHR regulates ASNS expression in an ATF4-dependent manner and affects MMP expression and activity. Target gene expression of three independent clones of H1975 expressing shAHR (K1-K3) and H1975 shScr control cells (shScr) was analyzed after treatment with omeprazole (omep, 200 uM, 48 h) or DMSO (-) by qRT-PCR ( a , b ) and immunoblotting ( c - e ). a qRT-PCR analysis of ASNS mRNA expression. b qRT-PCR analysis of MMP24 mRNA expression. c - e Immunoblotting analysis of AHR, ASNS ( c ), MMP24 ( d ), and ATF4 ( e ) protein expression. f Prior to treatment with omeprazole (omep, 200 uM, 48 h), H1975, A549, and H1299 were transfected with siRNAs targeting ATF4 or a non-targeting control (siCtrl). Expression of ASNS and ATF4 was assessed using immunoblotting. g qRT-PCR analysis of ASNS and ATF4 mRNA levels relative to siCtrl control. Target gene expression was normalized to two ( ACTB , GAPDH ) or three ( ACTB , GAPDH , HPRT1 ) housekeeping genes (HKG) as indicated. h Gelatine zymography of MMP2 and MMP9 activity. i Semiquantitative analysis of gelatinolytic bands from MMP9 (ImageJ). Data are shown as mean +- SD. Significance was assessed using one-way ANOVA.