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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- MAB10482 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB10482, RRID:AB_11188399
- Product name
- ACTA2 monoclonal antibody, clone 4A4
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against synthetic peptide of ACTA2.
- Isotype
- IgG
- Antibody clone number
- 4A4
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis using ACTA2 monoclonal antibody, clone 4A4 (Cat # MAB10482) against HeLa (1), A-431 (2), Jurkat (3), K-562 (4), HEK293 (5), HepG2 (6), NIH/3T3 (7), PC-12 (8) and COS-7 (9) cell lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- ELISA detection with ACTA2 monoclonal antibody, clone 4A4 (Cat # MAB10482).
- Validation comment
- ELISA
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HepG2 cells using ACTA2 monoclonal antibody, clone 4A4 (Cat # MAB10482) (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human duodenum tissues (A) and human esophagus tissues (B) using ACTA2 monoclonal antibody, clone 4A4 (Cat # MAB10482) with DAB staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HeLa cells using ACTA2 monoclonal antibody, clone 4A4 (Cat # MAB10482) (green) and negative control (purple).
- Validation comment
- Flow Cytometry
