Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- OAEB02335 - Provider product page
- Provider
- Aviva Systems Biology
- Proper citation
- Aviva Systems Biology Cat#OAEB02335, RRID:AB_10896114
- Product name
- ACTA2 Antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Horse, Porcine
- Host
- Goat
- Vial size
- 0.1mg
- Storage
- Aliquot and store at -20°C. Minimize freezing and thawing.
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Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- HEK293 lysate (10ug protein in RIPA buffer) over expressing Human ACTA2 with DYKDDDDK tag probed with OAEB02335 (1ug/ml,) in Lane A and probed with anti- DYKDDDDK Tag (1/3000) in lane C. Mock-transfected HEK293 probed with OAEB02335 (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.
- Sample type
- Human ACTA2
- Primary Ab dilution
- 1µg/mL
- Protocol
- Protocol
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- OAEB02335 staining (0.1µg/ml) of Human Duodenum lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
- Sample type
- Human Duodenum lysate
- Primary Ab dilution
- 0.1 µg/mL
- Protocol
- Protocol