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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- V2001 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Smooth Muscle Actin Antibody
- Antibody type
- Monoclonal
- Description
- This highly specific Smooth Muscle Actin antibody is suitable for use in Flow cytometry/Immunofluorescence/Immunohistochemistry/Western blot applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 1A4
- Vial size
- 20 ug (with BSA and sodium azide), 100 ug (with BSA and sodium azide), 100 ug (without BSA or sodium azide), 7 ml IHC only format (if applicable)
- Concentration
- 0.2 mg/ml, 1 mg/ml
- Storage
- Store the Smooth Muscle Actin antibody at 2-8oC (aliquot/freeze the azide-free format).
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human uterus lysate with Smooth muscle actin antibody (clone 1A4).
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of mouse NIH 3T3 cell lysate with Smooth Muscle Actin antibody.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of fixed human HeLa cells with Smooth Muscle Actin antibody (clone 1A4, green) and NucSpot nuclear stain (red).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC: human leiomyosarcoma stained with Smooth muscle actin antibody (1A4).
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC: FFPE human leiomyosarcoma stained with Smooth Muscle Actin antibody (clone 1A4).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of fixed human HeLa cells with Smooth Muscle Actin antibody (clone 1A4); Red=isotype control, Blue= Smooth Muscle Actin antibody.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE analysis of purified, BSA-free Smooth Muscle Actin antibody (clone 1A4) as confirmation of integrity and purity.
