Ab088443

antibody from Aladdin Scientific

Targeting: ACTA2 ACTSA

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

Ab088443

Provider

Aladdin Scientific

Product name

alpha-Smooth Muscle Actin Mouse mAb

Antibody type

Monoclonal

Description

Mouse anti Human Alpha smooth muscle Actin Antibody, Monoclonal (F1), could be used for WB, IHC and so on. ApplicationWB:0.001-2 _g/mLIHC: 5-20 µg/mLProtein FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Reactivity

Human, Mouse, Rat, Bovine, Canine, Porcine

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

AA 167-377


Antibody clone number

F1

Vial size

100_l,10_l,1ml,20_l,50_l

Concentration

Lot by Lot

Storage

Store at 4_ short term (1-2 weeks). Store at -20_ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Western blot

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Alpha smooth muscle Actin Mouse mAb (Ab088443) - Western Blot All lanes: Alpha smooth muscle Actin Mouse mAb (Ab088443) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/50000 dilution Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 5.6 s

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Alpha smooth muscle Actin Mouse mAb (Ab088443) - Western Blot All lanes: Alpha smooth muscle Actin Mouse mAb (Ab088443) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 26.7 s

Immunohistochemistry

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Alpha smooth muscle Actin Mouse mAb (Ab088443) - IHC DAB staining on IHC Sample: Human Ovarian cancer Tissue Primary Ab:10ug/mL Alpha smooth muscle Actin Mouse mAb (Ab088443) Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Alpha smooth muscle Actin Mouse mAb (Ab088443) - IHC DAB staining on IHC Sample: Human Rectum Tissue Primary Ab:10ug/mL Alpha smooth muscle Actin Mouse mAb (Ab088443) Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Alpha smooth muscle Actin Mouse mAb (Ab088443) - IHC DAB staining on IHC Sample: Rat Skeletal muscle Tissue Primary Ab: 3ug/mL Alpha smooth muscle Actin Mouse mAb (Ab088443) Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Alpha smooth muscle Actin Mouse mAb (Ab088443) - IHC DAB staining on IHC Sample: Rat Lung Tissue Primary Ab: 3ug/mL Alpha smooth muscle Actin Mouse mAb (Ab088443) Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Alpha smooth muscle Actin Mouse mAb (Ab088443) - IHC DAB staining on IHC Sample: Mouse Pancreas Tissue Primary Ab: 3ug/mL Alpha smooth muscle Actin Mouse mAb (Ab088443) Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Alpha smooth muscle Actin Mouse mAb (Ab088443) - IHC DAB staining on IHC Sample: Mouse Spleen Tissue Primary Ab: 3ug/mL Alpha smooth muscle Actin Mouse mAb (Ab088443) Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody