Ab088446

antibody from Aladdin Scientific

Targeting: ACTA2 ACTSA

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

Ab088446

Provider

Aladdin Scientific

Product name

Recombinant ACTA2 Antibody

Antibody type

Recombinant

Description

Rabbit anti Human ACTA2 Antibody, Recombinant (RR645), could be used for WB, IHC, Flow, IF, ICC and so on.Application:WB: 1/1000 - 1/2000 IHC: 1/2000 - 1/4000Flow: 1/20 - 1/100IF/ICC: 1/80 - 1/250

Reactivity

Human, Mouse, Rat, Bovine, Canine, Porcine, Zebrafish

Host

Rabbit

Conjugate

Unconjugated

Antibody clone number

RR645

Vial size

100_l,10_l,1ml,50_l

Concentration

0,05 mg/ml

Storage

Store at 4¡C short term (1-2 weeks). Store at -20¡C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Western blot

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Recombinant ACTA2 Antibody (Ab088446) - Western Blot All lanes: Recombinant ACTA2 Antibody (Ab088446) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 42 kDa Observed band size: 44 kDa Exposure time: 80.0 s

Immunocytochemistry

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Recombinant ACTA2 Antibody (Ab088446) - IF IF analysis of ACTA2 (green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained Recombinant ACTA2 Antibody (Ab088446) at 1/100 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

Immunohistochemistry

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Recombinant ACTA2 Antibody (Ab088446) - IHC Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of placenta tissue labelling ACTA2 with Recombinant ACTA2 Antibody (Ab088446) at 1/2000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.

Flow cytometry

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Recombinant ACTA2 Antibody (Ab088446) - Flow Cytometry Overlay histogram showing HeLa cells stained with Recombinant ACTA2 Antibody (Ab088446) (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in Recombinant ACTA2 Antibody (Ab088446) (1/100 dilution) in 1x PBS/1% BSA for 30 min at 4°C. The secondary antibody used was a Goat Anti-Rabbit Alexa Fluor® 488 (IgG H+L) at 1/2000 dilution for 20 min at 4°C. Unlabeled sample (Green) was used as a control.