AM06647SU-N

antibody from Acris Antibodies GmbH

Targeting: ACTA2 ACTSA

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AM06647SU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AM06647SU-N, RRID:AB_11214584

Product name

anti ACTA2 / aortic smooth muscle Actin

Antibody type

Monoclonal

Antigen

Synthesized peptide of human ACTA2.

Reactivity

Human, Mouse, Rat, Simian

Host

Mouse

Isotype

IgG

Antibody clone number

4A4

Vial size

0.1 ml

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot analysis using ACTA2 mouse mAb against Hela (1), A431 (2), Jurkat (3), K562 (4), HEK293 (5), HepG2 (6), NIH/3T3 (7), PC-12 (8) and Cos7 (9) cell lysate.

ELISA

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Red: Control Antigen (100ng)Purple: Antigen (10ng)Green: Antigen (50ng)Blue: Antigen (100ng)

Immunocytochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunofluorescence analysis of HepG2 cells using ACTA2 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human duodenum tissues (left) and human esophagus tissues (right) using ACTA2 mouse mAb with DAB staining.

Flow cytometry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunofluorescence analysis of HepG2 cells using ACTA2 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.