H00005528-M01

antibody from Abnova Corporation

Targeting: PPP2R5D B56D, B56delta

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00005528-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00005528-M01, RRID:AB_1579038

Product name

PPP2R5D monoclonal antibody (M01), clone 4E12

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full-length recombinant PPP2R5D.

Antigen sequence

MPYKLKKEKEPPKVAKCTAKPSSSGKDGGGENTEE
AQPQPQPQPQPQAQSQPPSSNKRPSNSTPPPTQLS
KIKYSGGPQIVKKERRQSSSRFNLSKNRELQKLPA
LKDSPTQEREELFIQKLRQCCVLFDFVSDPLSDLK
FKEVKRAGLNEMVEYITHSRDVVTEAIYPEAVTMF
SVNLFRTLPPSSNPTGAEFDPEEDEPTLEAAWPHL
QLVYEFFLRFLESPDFQPNIAKKYIDQKFVLALLD
LFDSEDPRERDFLKTILHRIYGKFLGLRAYIRRQI
NHIFYRFIYETEHHNGIAELLEILGSIINGFALPL
KEEHKMFLIRVLLPLHKVKSLSVYHPQLAYCVVQF
LEKESSLTEPVIVGLLKFWPKTHSPKEVMFLNELE
EILDVIEPSEFSKVMEPLFRQLAKCVSSPHFQVAE
RALYYWNNEYIMSLISDNAARVLPIMFPALYRNSK
SHWNKTIHGLIYNALKLFMEMNQKLFDDCTQQYKA
EKQKGRFRMKEREEMWQKIEELARLNPQYPMFRAP
PPLPPVYSMETETPTAEDIQLLKRTVETEAVQMLK
DIKKEKVLLRRKSELPQDVYTIKALEAHKRAEEFL
TASQEAL


Isotype

IgG

Antibody clone number

4E12

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of PPP2R5D expression in transfected 293T cell line by PPP2R5D monoclonal antibody (M01), clone 4E12.Lane 1: PPP2R5D transfected lysate(70 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged PPP2R5D is 1 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol