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Western blot
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- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
- Flow cytometry [2]
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- Product number
- RQ8110 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- PPP2R5D Antibody
- Antibody type
- Polyclonal
- Description
- This highly specific PPP2R5D antibody is suitable for use in Western blot/Flow cytometry/Immunofluorescence/Immunoprecipitation/Immunohistochemistry/Direct ELISA applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the PPP2R5D antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human HeLa, 2) human MCF7, 3) human HepG2, 4) rat brain, 5) rat PC-12, 6) mouse brain and 7) mouse Neuro-2a cell lysate with PPP2R5D antibody. Predicted molecular weight ~58 kDa, ~66 kDa and ~70 kDa (multiple isoforms).
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human HepG2, 2) human 293T, 3) human Jurkat, 4) rat brain and 5) mouse brain lysate with PPP2R5D antibody. Predicted molecular weight ~58 kDa, ~66 kDa and ~70 kDa (multiple isoforms).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE human U-2 OS cells with PPP2R5D antibody (green) and Alpha Tubulin mAb (red). HIER: steam section in pH6 citrate buffer for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunoprecipitation of PPP2R5D protein from 500ug of human HepG2 whole cell lysate with 2ug of PPP2R5D antibody.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human stomach cancer tissue with PPP2R5D antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of fixed and permeabilized human SiHa cells with PPP2R5D antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PPP2R5D antibody.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of fixed and permeabilized human 293T cells with PPP2R5D antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PPP2R5D antibody.
