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Western blot
Other assayAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Other assay [1]
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- Product number
- MA1-517 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Mist1 Monoclonal Antibody (6E8/A12/C11P1)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- MA1-517 detects Mist1 from mouse and human samples. MA1-517 has been successfully used in Western blot, immunohistochemistry procedures. The antibody reactivity in IP and ChIP is weaker than in other applications. By Western blot, MA1-517 detects a ~29 kDa band representing Mist1. The MA1-517 immunogen is mouse Mist1, C-terminus residues 175-197.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 6E8/A12/C11P1
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Pancreatic stromal Gremlin 1 expression during pancreatic tumorigenesis.
Deleterious Effects of SARS-CoV-2 Infection on Human Pancreatic Cells.
Davis JM, Cheng B, Drake MM, Yu Q, Yang B, Li J, Liu C, Younes M, Zhao X, Bailey JM, Shen Q, Ko TC, Cao Y
Genes & diseases 2022 Jan;9(1):108-115
Genes & diseases 2022 Jan;9(1):108-115
Deleterious Effects of SARS-CoV-2 Infection on Human Pancreatic Cells.
Shaharuddin SH, Wang V, Santos RS, Gross A, Wang Y, Jawanda H, Zhang Y, Hasan W, Garcia G Jr, Arumugaswami V, Sareen D
Frontiers in cellular and infection microbiology 2021;11:678482
Frontiers in cellular and infection microbiology 2021;11:678482
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1 Expression of ACE2 and TMPRSS2 in exocrine pancreatic cells. (A) iPan EXO Ductal cells CK19 (red) exhibiting i . ACE2 (green) and ii. TMPRSS2 (green) expression. (B) iPan EXO Acinar cells Amylase (red), MIST1 (gray) exhibit i . ACE2 (green) expression and ii. iPan EXO Acinar CTRC (red) exhibit TMPRSS2 (green). (C) iPan EXO Acinar and iPan ENDO cultures contain some endocrine C-peptide expressing cells, which also co-stain with ACE2. (D) iPSC-derived pancreatic exocrine cells as well as human acinar tissues and human ductal cell line H6C7 express ACE2 and TMPRSS2 . Data is shown as mean +- SEM with statistical significance determined by unpaired two-tailed t-test. **p < 0.01 and ****p < 0.0001. Scale bar represents 100 um, and 20 um for zoomed panels adjacent to main images. ICC images shown here are representative results from 27 independent sites acquired. RNA was extracted from aggregates of 2-3 biological replicates (using 12 well-plates), and qPCR was run with 3 technical replicates per sample. These results were pooled from 2 independent rounds of infection experiments.
