PA1-1961

antibody from Invitrogen Antibodies

Targeting: PSME4 KIAA0077, PA200

Western blot (Data presented on provider website)

Immunoprecipitation (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-1961

Provider

Invitrogen Antibodies

Product name

PSME4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

PA1-1961 detects PA200 from bovine, human, mouse and rat samples. PA1-1961 has been successfully used in Western blot, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody detects an ~200 kDa protein representing PA200 from bovine testis tissue extract. In mouse tissues, this antibody detects both a 200 kDa and a 160 kDa protein. In mouse tissues, the 200 kDa species is most abundant in testis whereas the 160 kDa species is enriched in the spleen. Immunofluorescence staining of PA200 in HeLa cells results in diffuse nuclear staining prior to ionizing radiation treatment. Immunofluorescence staining of PA200 in HeLa cells after ionizing radiation treatment results in focal aggregate staining within the nucleus. The PA1-1961 immunogen is a synthetic peptide corresponding to residues T(1620) K L P K K R K R D P G S V G(1634) of human PA200.

Reactivity

Human, Mouse, Rat, Bovine

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

Conc. Not Determined

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

The proteasome activator PA200 regulates expression of genes involved in cell survival upon selective mitochondrial inhibition in neuroblastoma cells.

Douida A, Batista F, Robaszkiewicz A, Boto P, Aladdin A, Szenykiv M, Czinege R, Virág L, Tar K
Journal of cellular and molecular medicine 2020 Jun;24(12):6716-6730

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cAMP-induced phosphorylation of 26S proteasomes on Rpn6/PSMD11 enhances their activity and the degradation of misfolded proteins.

Lokireddy S, Kukushkin NV, Goldberg AL
Proceedings of the National Academy of Sciences of the United States of America 2015 Dec 29;112(52):E7176-85

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PA200, a nuclear proteasome activator involved in DNA repair.

Ustrell V, Hoffman L, Pratt G, Rechsteiner M
The EMBO journal 2002 Jul 1;21(13):3516-25

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

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Experimental details

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Supportive validation

Submitted by

Invitrogen Antibodies (provider)

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Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

4 FIGURE PA200 occurrence in the genome of SH-SY5Y responds to cell treatment with mitochondria-impairing agents. (A) PA200 distribution around gene transcription start sites (TSS) was monitored by analysing mapped ChIP-seq reads with deepTools. (B) and (C) Peak calling in MACS revealed PA200 presence at gene promoters and their biological function, analysed with Amigo2 (B), disclosed crucial cellular processes. (C) Some PA200-positive gene promoters were simultaneously characterized by histone acetylation (H3K27ac) that marks actively transcribed genes. (D) Venn diagrams display PA200-enriched active (+H3K27ac) and inactive (-H3K27ac) gene promoters. (E) PA200 association with chromatin after cell treatment with 10 mumol/L rotenone, 3 mumol/L oligomycin and 100 nmol/L antimycin A was monitored and quantified by bedCoverage. (F) Alterations in the occupancy of gene promoters by PA200 in untreated cells and cells challenged with mitochondria-impairing agents were compared by Venn diagrams

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Proteasome activation by purified bovine PA200. Purified PA200 or 20S proteasomes were analyzed separately or mixed prior to electrophoresis on a native gel. Peptide overlay (left) demonstrated that PA200 activated the proteasome and western blotting (right) confirmed that some of the added PA200 bound the 20S proteasome (denoted 20S-PA200) in the right panel.