Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AF7039 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Tissue alpha-L-Fucosidase/FUCA1 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Tissue alpha-L-Fucosidase/FUCA1 in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
P04066
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Tissue alpha-L-Fucosidase/FUCA1 by Western Blot. Western blot shows lysates of human thyroid tissue and human kidney tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Tissue alpha-L-Fucosidase/FUCA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7039) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Tissue alpha-L-Fucosidase/FUCA1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Tissue alpha-L-Fucosidase/FUCA1 in HeLa Human Cell Line. Tissue alpha-L-Fucosidase/FUCA1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human Tissue alpha-L-Fucosidase/FUCA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7039) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to lysosomes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Tissue alpha-L-Fucosidase/FUCA1 in Human Liver. Tissue alpha-L-Fucosidase/FUCA1 was detected in immersion fixed paraffin-embedded sections of human liver using Sheep Anti-Human Tissue alpha-L-Fucosidase/FUCA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7039) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Sheep IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC006). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lysosomes in hepatocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.