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ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [3]
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- Product number
- PA5-90023 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NFE2L1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: SSSFSEEGAV GYSSDSETLD LEEAEGAVGY QPEYSKFCRM SYQDPAQLSC LPYLEHVGHN HTYNMAPSAL DSADLPPPSA LKKGSKEKQA DFLDKQMSRD EHRARAMKIP FTNDKIINLP VEEFNELLSK YQLSEAQLSL IRDIRRRGKN KMAAQNCRKR KLDTILNLER DVEDLQRDKA RLLREKVEFL RSLRQMKQKV QSLYQEVFGR LRDENGRPYS PSQYALQYAG DGSVLLIPRT MADQQARRQE RKPKDRRK; Positive Samples: HepG2, Jurkat, PC-12, Mouse fat, Mouse lung, Mouse liver; Cellular Location: Endoplasmic reticulum membrane, Nucleus, Single-pass type II membrane protein
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.894 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NFE2L1 in extracts of various cell lines. Samples were incubated with NFE2L1 Polyclonal antibody (Product # PA5-90023) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of NFE2L1 was performed in HeLa cells using NFE2L1 Polyclonal Antibody (Product # PA5-90023).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of NFE2L1 in HeLa cells. Samples were incubated with NFE2L1 Polyclonal antibody (Product # PA5-90023) using a dilution of 1:100. Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of NFE2L1 in paraffin-embedded rat heart. Samples were incubated with NFE2L1 Polyclonal antibody (Product # PA5-90023) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of NFE2L1 in paraffin-embedded human placenta. Samples were incubated with NFE2L1 Polyclonal antibody (Product # PA5-90023) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of NFE2L1 in paraffin-embedded mouse brain. Samples were incubated with NFE2L1 Polyclonal antibody (Product # PA5-90023) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
