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Western blot
Immunocytochemistry
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- HPA048020 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA048020, RRID:AB_2680234
- Product name
- Anti-TOMM70
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human TOMM70, Gene description: translocase of outer mitochondrial membrane 70, Alternative Gene Names: KIAA0719, Tom70, TOMM70A, Validated applications: WB, IHC, ICC, Uniprot ID: O94826, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.2 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references OCIAD1 and prohibitins regulate the stability of the TIM23 protein translocase
The Orf9b protein of SARS-CoV-2 modulates mitochondrial protein biogenesis
The typeIIIsecretion system effectorEspOof enterohaemorrhagicEscherichia coliinhibits apoptosis through an interaction withHAX‐1
Elancheliyan P, Maruszczak K, Serwa R, Stephan T, Gulgec A, Borrero-Landazabal M, Ngati S, Gosk A, Jakobs S, Wasilewski M, Chacinska A
Cell Reports 2024;43(12):115038
Cell Reports 2024;43(12):115038
The Orf9b protein of SARS-CoV-2 modulates mitochondrial protein biogenesis
Lenhard S, Gerlich S, Khan A, Rödl S, Bökenkamp J, Peker E, Zarges C, Faust J, Storchova Z, Räschle M, Riemer J, Herrmann J
Journal of Cell Biology 2023;222(10)
Journal of Cell Biology 2023;222(10)
The typeIIIsecretion system effectorEspOof enterohaemorrhagicEscherichia coliinhibits apoptosis through an interaction withHAX‐1
Chatterjee S, Lekmeechai S, Constantinou N, Grzybowska E, Kozik Z, Choudhary J, Berger C, Frankel G, Clements A
Cellular Microbiology 2021;23(9)
Cellular Microbiology 2021;23(9)
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Enhanced validation
- Submitted by
- klas2
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot of cell lysate from U-2 OS cells transfected with either siRNA targeting TOMM70A or control siRNA. Lane 1: Marker (250, 130, 95, 72, 55, 36, 28, 17, 10) Lane 2: Cell lysate from U-2OS cells transfected with siRNA targeting TOMM70A Lane 3: N/A Lane 4: Cell lysate from U-2OS cells transfected with control siRNA Right image, lane 1-4: loading control
- Sample type
- U-2 OS
- Primary Ab dilution
- 1:210
- Conjugate
- Horseradish Peroxidase
- Secondary Ab
- Secondary Ab
- Secondary Ab dilution
- 1:3000
- Knockdown/Genetic Approaches Application
- Western blot
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.
- Sample type
- Human
