14528-1-AP

antibody from Invitrogen Antibodies

Targeting: TOMM70 KIAA0719, Tom70, TOMM70A

Provider product page for 14528-1-AP

Western blot

Immunoprecipitation

Antibody data

Antibody Data
Antigen structure
References [0]
Comments [0]
Validations
Western blot [9]
Immunocytochemistry [2]
Immunohistochemistry [8]
Flow cytometry [1]
Other assay [1]

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Product number: 14528-1-AP - Provider product page
Provider: Invitrogen Antibodies
Product name: TOM70 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Other
Description: Immunogen sequence: KNREPLMPS PQFIKSYFSS FTDDIISQPM LKGEKSDEDK DKEGEALEVK ENSGYLKAKQ YMEEENYDKI ISECSKEIDA EGKYMAEALL LRATFYLLIG NANAAKPDLD KVISLKEANV KLRANALIKR GSMYMQQQQP LLSTQDFNMA ADIDPQNADV YHHRGQLKIL LDQVEEAVAD FDECIRLRPE SALAQAQKCF ALYRQAYTGN NSSQIQAAMK GFEEVIKKFP RCAEGYALYA QALTDQQQFG KADEMYDKCI DLEPDNATTY VHKGLLQLQW KQDLDRGLEL ISKAIEIDNK CDFAYETMGT IEVQRGNMEK AIDMFNKAIN LAKSEMEMAH LYSLCDAAHA QTEVAKKYGL KPPTL (245-608 aa encoded by BC052994)
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 150 µL
Concentration: 0.26 mg/mL
Storage: -20°C

TOMM70 protein structure - 14528-1-AP shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Mouse liver tissue were subjected to SDS PAGE followed by western blot with 14528-1-AP (TOM70 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Mouse heart tissue were subjected to SDS PAGE followed by western blot with 14528-1-AP (TOM70 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: WB result of TOM70 antibody (14528-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TOM70 transfected HeLa cells.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14528-1-AP (TOM70 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: MCF7 cells were subjected to SDS PAGE followed by western blot with 14528-1-AP (TOM70 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Mouse brain tissue were subjected to SDS PAGE followed by western blot with 14528-1-AP (TOM70 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Mouse liver mitochondrial lysate was subjected to SDS PAGE followed by western blot with 14528-1-AP (TOM70 Antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Rat heart tissue were subjected to SDS PAGE followed by western blot with 14528-1-AP (TOM70 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14528-1-AP (TOM70 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human kidney tissue slide using 14528-1-AP (TOM70 Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human kidney tissue slide using 14528-1-AP (TOM70 Antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded mouse heart tissue slide using 14528-1-AP (TOM70 antibody) at dilution of 1:400 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded mouse heart tissue slide using 14528-1-AP (TOM70 antibody) at dilution of 1:400 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human liver using 14528-1-AP (TOM70 antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human liver using 14528-1-AP (TOM70 antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human heart using 14528-1-AP (TOM70 antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human heart using 14528-1-AP (TOM70 antibody) at dilution of 1:50 (under 40x lens).