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Western blot
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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [2]
- Immunoprecipitation [1]
- Other assay [1]
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- Product number
- PA5-88357 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NDUFA8 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: GAQCDKPNKE FMLCRWEEKD PRRCLEEGKL VNKCALDFFR QIKRHCAEPF TEYWTCIDYT GQQLFRHCRK QQAKFDECVL DKLGWVRPDL GELSKVTKVK TDRPLPENPY HSRPRPDPSP EIEGDLQPAT HGSRFYFWTK; Positive Samples: 293T, LO2, MCF7, Mouse heart, Mouse brain, Rat heart; Cellular Location: Mitochondrion inner membrane, Mitochondrion intermembrane space, Peripheral membrane protein
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 11.28 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of NDUFA8 in H9C2 cells. Samples were incubated with NDUFA8 Polyclonal antibody (Product # PA5-88357) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of NDUFA8 in L929 cells. Samples were incubated with NDUFA8 Polyclonal antibody (Product # PA5-88357) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of NDUFA8 in 200 μg extracts of 293T cells. Samples were precipitated with 3 μg NDUFA8 Polyclonal antibody (Product # PA5-88357). Western blot was performed from the immunoprecipitate using NDUFA8 Polyclonal antibody (Product # PA5-88357) at a dilution of 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of NDUFA8 was performed in 200 µg extracts of 293T cells using NDUFA8 Polyclonal Antibody (Product # PA5-88357). Western blot was performed from the immunoprecipitate using NDUFA8 Polyclonal Antibody.
