19893-1-AP

antibody from Proteintech Group

Targeting: RFWD3 FANCW, FLJ10520, RNF201

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

19893-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#19893-1-AP, RRID:AB_10639521

Product name

RFWD3 antibody

Antibody type

Polyclonal

Description

RFWD3 antibody (Cat. #19893-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Ski2-like helicase ASCC3 unwinds DNA upon fork stalling to control replication stress responses.

Cui S, Batenburg NL, Coulombe Y, Arumugam A, Walker JR, Sheida SV, Bielinsky AK, Wahl MC, Masson JY, Zhu XD
Cell reports 2026 Mar 24;45(3):117051

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The Ski2 helicase ASCC3 unwinds DNA upon fork stalling to control replication stress responses.

Cui S, Batenburg NL, Coulombe Y, Arumugam A, Walker JR, Sheida SV, Bielinsky AK, Wahl MC, Masson JY, Zhu XD
bioRxiv : the preprint server for biology 2025 Jul 26;

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RFWD3 Reprograms Nucleotide Metabolism Through PHGDH to Induce Chemoresistance In Osteosarcoma.

Zhang W, Yin C, Qi L, Liu Z, Xu R, Tu C, Li Z
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2025 Apr;12(16):e2410937

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RFWD3 modulates response to platinum chemotherapy and promotes cancer associated phenotypes in high grade serous ovarian cancer.

Taylor SJ, Hollis RL, Gourley C, Herrington CS, Langdon SP, Arends MJ
Frontiers in oncology 2024;14:1389472

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RFWD3 acts as a tumor promotor in the development and progression of bladder cancer.

Jiang P, Xu Z, Wu S, Sun J, Tian J, Chen X
Histology and histopathology 2023 Aug;38(8):917-928

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The Valproate Mediates Radio-Bidirectional Regulation Through RFWD3-Dependent Ubiquitination on Rad51.

Liu G, Lim D, Cai Z, Ding W, Tian Z, Dong C, Zhang F, Guo G, Wang X, Zhou P, Feng Z
Frontiers in oncology 2021;11:646256

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DOCK7 protects against replication stress by promoting RPA stability on chromatin.

Gao M, Guo G, Huang J, Hou X, Ham H, Kim W, Zhao F, Tu X, Zhou Q, Zhang C, Zhu Q, Liu J, Yan Y, Xu Z, Yin P, Luo K, Weroha J, Deng M, Billadeau DD, Lou Z
Nucleic acids research 2021 Apr 6;49(6):3322-3337

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Identification of an E3 ligase-encoding gene RFWD3 in non-small cell lung cancer.

Zhang Y, Zhao X, Zhou Y, Wang M, Zhou G
Frontiers of medicine 2020 Jun;14(3):318-326

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

mouse lung tissue were subjected to SDS PAGE followed by western blot with 19893-1-AP(RFWD3 antibody) at dilution of 1:1000

Sample type

tissue

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using RFWD3 antibody 19893-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human testis using 19893-1-AP(RFWD3 antibody) at dilution of 1:400 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human testis using 19893-1-AP(RFWD3 antibody) at dilution of 1:400 (under 40x lens)

Sample type

tissue