15201-1-AP

antibody from Proteintech Group

Targeting: IFIT3 CIG-49, GARG-49, IFI60, IFIT4, IRG2, ISG60, RIG-G

Provider product page for 15201-1-AP

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Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [59]
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Validations
Western blot [1]
Immunoprecipitation [1]

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Product number: 15201-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#15201-1-AP, RRID:AB_2248738
Product name: IFIT3 antibody
Antibody type: Polyclonal
Description: KD/KO validated IFIT3 antibody (Cat. #15201-1-AP) is a rabbit polyclonal antibody that shows reactivity with human and has been validated for the following applications: IF, IHC, IP, WB, ELISA.
Reactivity: Human
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

IFIT3 protein structure - 15201-1-AP shown in red.

Submitted references Betrixaban activates cGAS and ERVs to promote dual nucleic-sensing antiviral immunity.

Chen X, Zhao Y, Xie Y, Liu T, Wang H, Wang X, Guo X, You F
EMBO molecular medicine 2026 May;18(5):1563-1591

Integrative Analyses Identify a cGAS-STING Pathway-Driven Signature With Context-Dependent Roles in Systemic Lupus Erythematosus.

Zhang L, Lyu MA, Hong Z, Sun J, Guo L, Hu J, Maimaiti M, Li C, Song J, Zhang X, Yu H, Wang C, Wang X, Hu H
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2026 May;13(25):e21560

The segmented flavivirus ALSV-encoded nucleoprotein VP2 inhibits type I interferon production by targeting RIG-I.

Pan M, Song Z, Wang M, Zhao M, Fang S, Jin F, Tan Q, Xu W, Che L, Liu N, Sui L, Liu Q, Hou Z, Zhao Y
Microbiology spectrum 2026 Mar 3;14(3):e0248425

VILMIR is a trans-acting long noncoding RNA that enhances the host interferon response in human epithelial cells.

John K, Smith E, Istishin A, Mahmood N, Diveley K, Tollison TS, Carpenter S, Peng X
Journal of virology 2026 Jan 20;100(1):e0138025

The promoting roles of GLP1R and GIPR in stemness maintenance and multiple lineage-specific differentiation of PDLSCs.

Shen Y, Zhang M, Yang T, Wu Y, Qiu Y, Zhang L, Li F, Chen M, Chen Q, Wei W, Li H, Shen Y
Cellular & molecular biology letters 2026 Feb 11;31(1):22

Discovery and confirmation of crucial genes associated with radiation-induced heart disease.

Liu C, Shi J, Xing L, Yao B, Liu J, Wang Y, Fan J
International journal of medical sciences 2025;22(6):1278-1291

Downregulation of IFIT3 Relieves the Inflammatory Response in Ulcerative Colitis via Selectively Regulating Macrophage M1 Polarization and the STAT1/2 Signaling Pathway.

Du M, Tao Y, Yang K, Liu J, Yang X
Journal of inflammation research 2025;18:12295-12309

IFIT3 activation significantly contributes to HIV-1-associated neurodegenerative disorder-mediated neuroinflammation.

Das RK, Sahoo N, Roy D, Xu C, Galarza J, Garza MCM, Rodrigo H, Duttaroy AK, Roy U
Frontiers in immunology 2025;16:1532318

Single-cell atlas of human liver and blood immune cells across fatty liver disease stages reveals distinct signatures linked to liver dysfunction and fibrogenesis.

Martin OP, Wallace MS, Oetheimer C, Patel HB, Butler MD, Wong LP, Huang P, Elbaz J, Costentin C, Salloum S, Reinus Z, Obinelo A, Kim U, Shroff S, Corey KE, Popov YV, Charles ED, Sadreyev RI, Chung RT, Alatrakchi N
Nature immunology 2025 Sep;26(9):1596-1611

Dissecting the immune landscape in pediatric high-grade glioma reveals cell state changes under therapeutic pressure.

LaBelle JJ, Haase RD, Beck A, Haase J, Jiang L, Oliveira de Biagi CA Jr, Neyazi S, Englinger B, Liu I, Trissal M, Jeong D, Hack OA, Nascimento A, Shaw ML, Nguyen CM, Castellani S, Mathewson ND, Ashenberg O, Veiga Cruzeiro GA, Rosenberg T, Vogelzang JR, Pyrdol J, Marx S, Luomo AM, Godicelj A, Baumgartner A, Rozowsky JS, Madlener S, Mayr L, Peyrl A, Geyeregger R, Loetsch D, Dorfer C, Haberler C, Stepien N, Slavc I, Davidson TB, Prins RM, Yeo KK, Cooney T, Ligon K, Lidov H, Alexandrescu S, Baird LC, Gojo J, Wucherpfennig KW, Filbin MG
Cell reports. Medicine 2025 May 20;6(5):102095

Immunomodulatory roles of autophagic flux and IFIT in human ectocervical cells upon Trichomonas vaginalis infection.

Liu CC, Chu LJ, Yeh YM, Lin HC, Chen LC, Huang CY, Chiu SF, Cheng FW, Lin WN, Huang KY
International immunopharmacology 2025 May 16;155:114643

A poxvirus ankyrin protein LSDV012 inhibits IFIT1 in a host-species-specific manner by compromising its RNA binding ability.

Xie S, Fang Y, Liao Z, Cui L, Niu K, Ren S, Zhu J, Wu W, Jing Z, Peng C
PLoS pathogens 2025 Mar;21(3):e1012994

Acevaltrate overcomes myeloma resistance to bortezomib via pyroptosis by promoting BAX translocalization to mitochondria.

Wang Y, Cui Y, Liu Z, Liu L, Huang Z, Wang Q, Mao X
European journal of pharmacology 2025 Jun 5;996:177572

IFIT3 RNA-binding activity promotes influenza A virus infection and translation efficiency.

Sullivan OM, Nesbitt DJ, Schaack GA, Feltman EM, Nipper T, Kongsomros S, Reed SG, Nelson SL, King CR, Shishkova E, Coon JJ, Mehle A
Journal of virology 2025 Jul 22;99(7):e0028625

IFIT3 promotes lymph node metastasis by interacting with LASP1 to activate FAK-ERK signaling in esophageal squamous cell carcinoma.

Cui Q, Zhou X, Chen X, Zhao X, Zhang R, Wang J, Li X, Wang G, Li B, Li M, Chen J, Gao X, Shi Y, Li W, Hong P, Li D
Cell death & disease 2025 Dec 18;17(1):110

Silencing IFIT3 suppresses the EGFR/VEGF pathway and modulates SOCS1 to attenuate skin fibrosis in systemic sclerosis.

Huang X, Liu Y, Fu H, Rong X, Zhao Y
Apoptosis : an international journal on programmed cell death 2025 Aug;30(7-8):1852-1874

VILMIR is a trans-acting long noncoding RNA that enhances the host interferon response in human epithelial cells.

John K, Smith E, Istishin A, Mahmood N, Diveley K, Tollison TS, Carpenter S, Peng X
bioRxiv : the preprint server for biology 2025 Aug 22;

Porcine reproductive and respiratory syndrome virus nsp5 inhibits the activation of the Nrf2/HO-1 pathway by targeting p62 to antagonize its antiviral activity.

Wang F, Amona FM, Pang Y, Zhang Q, Liang Y, Chen X, Ke Y, Chen J, Song C, Wang Y, Li Z, Zhang C, Fang X, Chen X
Journal of virology 2025 Apr 15;99(4):e0158524

IFIT3 RNA-binding activity promotes influenza A virus infection and translation efficiency.

Sullivan OM, Nesbitt DJ, Schaack GA, Feltman E, Nipper T, Kongsomros S, Reed SG, Nelson SL, King CR, Shishkova E, Coon JJ, Mehle A
bioRxiv : the preprint server for biology 2025 Apr 12;

Machine Learning Screening and Validation of PANoptosis-Related Gene Signatures in Sepsis.

Xu J, Zhu M, Luo P, Gong Y
Journal of inflammation research 2024;17:4765-4780

ISG15/USP18/STAT2 is a molecular hub regulating IFN I-mediated control of Dengue and Zika virus replication.

Espada CE, da Rocha EL, Ricciardi-Jorge T, Dos Santos AA, Soares ZG, Malaquias G, Patrício DO, Gonzalez Kozlova E, Dos Santos PF, Bordignon J, Sanford TJ, Fajardo T, Sweeney TR, Báfica A, Mansur DS
Frontiers in immunology 2024;15:1331731

IFIT3 mediates TBK1 phosphorylation to promote activation of pDCs and exacerbate systemic sclerosis in mice.

Huang X, Liu Y, Rong X, Zhao Y, Feng D, Wang J, Xing W
Clinical and translational medicine 2024 Sep;14(9):e1800

Nuclear PCGF3 inhibits the antiviral immune response by suppressing the interferon-stimulated gene.

Da G, Wang J, Shang J, Xun C, Yu Y, Wang Y, Tie N, Li H
Cell death discovery 2024 Oct 5;10(1):429

Differential Effects of Extracellular Vesicles from Two Different Glioblastomas on Normal Human Brain Cells.

Wang M, Graner AN, Knowles B, McRae C, Fringuello A, Paucek P, Gavrilovic M, Redwine M, Hanson C, Coughlan C, Grimaldo-Garcia S, Metzger B, Bolus V, Kopper TJ, Smith M, Zhou W, Lenz M, Abosch A, Ojemann S, Lillehei KO, Yu X, Graner MW
Neurology international 2024 Nov 6;16(6):1355-1384

CK2 phosphorylation of CMTR1 promotes RNA cap formation and influenza virus infection.

Lukoszek R, Inesta-Vaquera F, Brett NJM, Liang S, Hepburn LA, Hughes DJ, Pirillo C, Roberts EW, Cowling VH
Cell reports 2024 Jul 23;43(7):114405

LRRK2 kinase activity restricts NRF2-dependent mitochondrial protection in microglia.

Weindel CG, Ellzey LM, Coleman AK, Patrick KL, Watson RO
bioRxiv : the preprint server for biology 2024 Jul 13;

Cell fusion upregulates PD-L1 expression for evasion from immunosurveillance.

Tajima Y, Shibasaki F, Masai H
Cancer gene therapy 2024 Jan;31(1):158-173

ETV7 promotes colorectal cancer progression through upregulation of IFIT3.

Chai B, Li Y, Guo Y, Zhang Z, Jia K, Chai X, Suo Y
Functional & integrative genomics 2024 Jan 10;24(1):8

Downregulation of CPSF6 leads to global mRNA 3' UTR shortening and enhanced antiviral immune responses.

Ge Y, Huang J, Chen R, Fu Y, Ling T, Ou X, Rong X, Cheng Y, Lin Y, Zhou F, Lu C, Yuan S, Xu A
PLoS pathogens 2024 Feb;20(2):e1012061

Porcine deltacoronavirus nsp5 antagonizes type I interferon signaling by cleaving IFIT3.

Huang H, Lei X, Zhao C, Qin Y, Li Y, Zhang X, Li C, Lan T, Zhao B, Sun W, Lu H, Jin N
Journal of virology 2024 Feb 20;98(2):e0168223

Knockdown of IFIT3 ameliorates multiple sclerosis via selectively regulating M1 polarization of microglia in an experimental autoimmune encephalomyelitis model.

Sun R, Wang YF, Yang X
International immunopharmacology 2024 Feb 15;128:111501

Induction of IFIT1/IFIT3 and inhibition of Bcl-2 orchestrate the treatment of myeloma and leukemia via pyroptosis.

He Y, Jiang S, Cui Y, Liang J, Zhong Y, Sun Y, Moran MF, Huang Z, He G, Mao X
Cancer letters 2024 Apr 28;588:216797

A tale of two tumors: differential, but detrimental, effects of glioblastoma extracellular vesicles (EVs) on normal human brain cells.

Wang M, Graner AN, Knowles B, McRae C, Fringuello A, Paucek P, Gavrilovic M, Redwine M, Hanson C, Coughlan C, Metzger B, Bolus V, Kopper T, Smith M, Zhou W, Lenz M, Abosch A, Ojemann S, Lillehei KO, Yu X, Graner MW
bioRxiv : the preprint server for biology 2024 Apr 12;

Shared biomarkers of multi-tissue origin for primary Sjogren's syndrome and their importance in immune microenvironment alterations.

Cai T, Xu J, Fang Y, Wu Y, Qin Q, Zhang JA
Immunobiology 2023 Sep;228(5):152726

Type I/type III IFN and related factors regulate JEV infection and BBB endothelial integrity.

Zhang YG, Zhang HX, Chen HW, Lv P, Su J, Chen YR, Fu ZF, Cui M
Journal of neuroinflammation 2023 Sep 27;20(1):216

EGR1 functions as a new host restriction factor for SARS-CoV-2 to inhibit virus replication through the E3 ubiquitin ligase MARCH8.

Zhao Y, Sui L, Wu P, Li L, Liu L, Ma B, Wang W, Chi H, Wang Z-D, Wei Z, Hou Z, Zhang K, Niu J, Jin N, Li C, Zhao J, Wang G, Liu Q
Journal of virology 2023 Oct 31;97(10):e0102823

Porcine Mx proteins inhibit pseudorabies virus replication through interfering with early gene synthesis.

Chen J, Hu JH, Sun RC, Li XH, Zhou J, Zhou B
Veterinary microbiology 2023 May;280:109706

Single-Cell Transcriptomic Analysis of Primary and Metastatic Tumor Ecosystems in Esophageal Squamous Cell Carcinoma.

Jia Y, Zhang B, Zhang C, Kwong DL, Chang Z, Li S, Wang Z, Han H, Li J, Zhong Y, Sui X, Fu L, Guan X, Qin Y
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2023 Mar;10(7):e2204565

SARS-CoV-2 ORF3a inhibits cGAS-STING-mediated autophagy flux and antiviral function.

Su J, Shen S, Hu Y, Chen S, Cheng L, Cai Y, Wei W, Wang Y, Rui Y, Yu XF
Journal of medical virology 2023 Jan;95(1):e28175

Hepatocytes demarcated by EphB2 contribute to the progression of nonalcoholic steatohepatitis.

Xiao Y, Batmanov K, Hu W, Zhu K, Tom AY, Guan D, Jiang C, Cheng L, McCright SJ, Yang EC, Lanza MR, Liu Y, Hill DA, Lazar MA
Science translational medicine 2023 Feb 8;15(682):eadc9653

TRAF3 activates STING-mediated suppression of EV-A71 and target of viral evasion.

Zheng W, Zhou Z, Rui Y, Ye R, Xia F, Guo F, Liu X, Su J, Lou M, Yu XF
Signal transduction and targeted therapy 2023 Feb 24;8(1):79

Citrullination profile analysis reveals peptidylarginine deaminase 3 as an HSV-1 target to dampen the activity of candidate antiviral restriction factors.

Pasquero S, Gugliesi F, Biolatti M, Dell'Oste V, Albano C, Bajetto G, Griffante G, Trifirò L, Brugo B, Raviola S, Lacarbonara D, Yang Q, Sudeshna S, Barasa L, Haniff H, Thompson PR, Landolfo S, De Andrea M
PLoS pathogens 2023 Dec;19(12):e1011849

The Influenza B Virus Victoria and Yamagata Lineages Display Distinct Cell Tropism and Infection Induced Host Gene Expression in Human Nasal Epithelial Cell Cultures.

Wilson JL, Akin E, Zhou R, Jedlicka A, Dziedzic A, Liu H, Fenstermacher KZ, Rothman R, Pekosz A
bioRxiv : the preprint server for biology 2023 Aug 4;

Proteomic Investigation Reveals Eukaryotic Translation Initiation Factor 5A Involvement in Porcine Reproductive and Respiratory Syndrome Virus Infection in vitro.

Li H, Wan B, Jiang D, Ji P, Zhao M, Li X, Li R, Qiao S
Frontiers in veterinary science 2022;9:861137

Systematic Analysis of Molecular Subtypes Based on the Expression Profile of Immune-Related Genes in Pancreatic Cancer.

Ke M
Oxidative medicine and cellular longevity 2022;2022:3124122

COL8A1 Promotes NSCLC Progression Through IFIT1/IFIT3-Mediated EGFR Activation.

Zan X, Li S, Wei S, Gao L, Zhao L, Yan X, Zhao Y, Shi J, Wang Y, Liu R, Zhang Y, Wan Y, Zhou Y
Frontiers in oncology 2022;12:707525

DestVI identifies continuums of cell types in spatial transcriptomics data.

Lopez R, Li B, Keren-Shaul H, Boyeau P, Kedmi M, Pilzer D, Jelinski A, Yofe I, David E, Wagner A, Ergen C, Addadi Y, Golani O, Ronchese F, Jordan MI, Amit I, Yosef N
Nature biotechnology 2022 Sep;40(9):1360-1369

LncRNA RP5-998N21.4 promotes immune defense through upregulation of IFIT2 and IFIT3 in schizophrenia.

Guo B, Jiang T, Wu F, Ni H, Ye J, Wu X, Ni C, Jiang M, Ye L, Li Z, Zheng X, Li S, Yang Q, Wang Z, Huang X, Zhao C
Schizophrenia (Heidelberg, Germany) 2022 Mar 1;8(1):11

Increased presence of nuclear DNAJA3 and upregulation of cytosolic STAT1 and of nucleic acid sensors trigger innate immunity in the ClpP-null mouse.

Maletzko A, Key J, Wittig I, Gispert S, Koepf G, Canet-Pons J, Torres-Odio S, West AP, Auburger G
Neurogenetics 2021 Oct;22(4):297-312

Murine Ifit3 restricts the replication of Rabies virus both in vitro and in vivo.

Chai B, Tian D, Zhou M, Tian B, Yuan Y, Sui B, Wang K, Pei J, Huang F, Wu Q, Lv L, Yang Y, Wang C, Fu Z, Zhao L
The Journal of general virology 2021 Jul;102(7)

Mapping the molecular signatures of diet-induced NASH and its regulation by the hepatokine Tsukushi.

Xiong X, Wang Q, Wang S, Zhang J, Liu T, Guo L, Yu Y, Lin JD
Molecular metabolism 2019 Feb;20:128-137

Progression of pathology in PINK1-deficient mouse brain from splicing via ubiquitination, ER stress, and mitophagy changes to neuroinflammation.

Torres-Odio S, Key J, Hoepken HH, Canet-Pons J, Valek L, Roller B, Walter M, Morales-Gordo B, Meierhofer D, Harter PN, Mittelbronn M, Tegeder I, Gispert S, Auburger G
Journal of neuroinflammation 2017 Aug 2;14(1):154

STAT1 and NF-κB Inhibitors Diminish Basal Interferon-Stimulated Gene Expression and Improve the Productive Infection of Oncolytic HSV in MPNST Cells.

Jackson JD, Markert JM, Li L, Carroll SL, Cassady KA
Molecular cancer research : MCR 2016 May;14(5):482-92

Herpes Simplex Virus 1 Tegument Protein UL41 Counteracts IFIT3 Antiviral Innate Immunity.

Jiang Z, Su C, Zheng C
Journal of virology 2016 Dec 15;90(24):11056-11061

MAVS-MKK7-JNK2 defines a novel apoptotic signaling pathway during viral infection.

Huang Y, Liu H, Li S, Tang Y, Wei B, Yu H, Wang C
PLoS pathogens 2014 Mar;10(3):e1004020

Poly(I:C) inhibits porcine reproductive and respiratory syndrome virus replication in MARC-145 cells via activation of IFIT3.

Zhang L, Liu J, Bai J, Du Y, Wang X, Liu X, Jiang P
Antiviral research 2013 Sep;99(3):197-206

Two-dimensional liquid chromatography-tandem mass spectrometry coupled with isobaric tags for relative and absolute quantification (iTRAQ) labeling approach revealed first proteome profiles of pulmonary alveolar macrophages infected with porcine reproductive and respiratory syndrome virus.

Lu Q, Bai J, Zhang L, Liu J, Jiang Z, Michal JJ, He Q, Jiang P
Journal of proteome research 2012 May 4;11(5):2890-903

Proteomic analysis of the lungs of mice infected with different pathotypes of H5N1 avian influenza viruses.

Zhao D, Liang L, Li Y, Liu L, Guan Y, Jiang Y, Chen H
Proteomics 2012 Jun;12(12):1970-82

IFN-induced TPR protein IFIT3 potentiates antiviral signaling by bridging MAVS and TBK1.

Liu XY, Chen W, Wei B, Shan YF, Wang C
Journal of immunology (Baltimore, Md. : 1950) 2011 Sep 1;187(5):2559-68

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HepG2 cells were subjected to SDS PAGE followed by western blot with 15201-1-AP(IFIT3 antibody) at dilution of 1:800
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The IFIT3 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human IFIT3. This antibody recognizes human antigen. The IFIT3 antibody has been validated for the following applications: ELISA, WB, IP analysis.
Appendix: 15201-1-AP.pdf

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