16588-1-AP

antibody from Proteintech Group

Targeting: PC PCB

Provider product page for 16588-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [61]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]
Immunohistochemistry [2]

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Product number: 16588-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#16588-1-AP, RRID:AB_1851513
Product name: Pyruvate Carboxylase antibody
Antibody type: Polyclonal
Description: KD/KO validated Pyruvate Carboxylase antibody (Cat. #16588-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

PC protein structure - 16588-1-AP shown in red.

Submitted references Mitochondrial control of glycerolipid synthesis by a PEP shuttle.

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Branched-chain α-keto acids impair glucose-stimulated insulin secretion in pancreatic β-cells under diabetes by reactivating the LDHA-lactate axis.

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Nature communications 2026 Mar 2;17(1)

Mitochondrial control of glycerolipid synthesis by a PEP shuttle.

Yamamuro T, Katoh D, Silva GM, Nishida H, Oikawa S, Higuchi Y, Wang D, Fujimoto M, Yoshida N, Li M, Shin J, Zhao Z, Yook JS, Sun L, Kajimura S
bioRxiv : the preprint server for biology 2026 Mar 10;

Allosteric activation of pyruvate carboxylase by Dracoflavan B(2) enhances endothelial energy metabolism to attenuate myocardial infarction.

Hong C, Li H, Liu RY, Wang R, Sun YJ, Huang YL, Wei TT, Tu PF, Zeng KW, Li J
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Icaritin Ameliorates Cisplatin-Induced Mitochondrial Metabolic Dysfunction-Associated Nephrotoxicity and Synergistically Potentiates Its Antitumor Efficacy.

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Ganoderic acid T, a novel activator of pyruvate carboxylase, exhibits potent anti-liver cancer activity.

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Melanoma-derived versican reactivates tumor-associated macrophages by upregulating pyruvate carboxylase through TLR2-MyD88-RelB axis under normoxia.

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Lactate promotes fatty acid oxidation by the tricarboxylic acid cycle and mitochondrial respiration in muscles of obese mice.

Park SY, Jung SR, Kim JY, Kim YW, Sung HK, Park SY, Doh KO, Koh JH
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The Changes of Amino-Acid Metabolism between Wheat and Rice during Early Growth under Flooding Stress.

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International journal of molecular sciences 2024 May 11;25(10)

The opposite role of lactate dehydrogenase a (LDHA) in cervical cancer under energy stress conditions.

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Cardiomyocyte-specific deletion of GCN5L1 reduces lysine acetylation and attenuates diastolic dysfunction in aged mice by improving cardiac fatty acid oxidation.

Stewart JE, Crawford JM, Mullen WE, Jacques A, Stoner MW, Scott I, Thapa D
The Biochemical journal 2024 Mar 20;481(6):423-436

Effects of endurance training under calorie restriction on energy substrate metabolism in mouse skeletal muscle and liver.

Takahashi K, Kitaoka Y, Hatta H
The journal of physiological sciences : JPS 2024 Jun 7;74(1):32

GPR30 selective agonist G-1 induced insulin resistance in ovariectomized mice on high fat diet and its mechanism.

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Biochemical and biophysical research communications 2024 Jul 5;716:150026

MCT1-governed pyruvate metabolism is essential for antibody class-switch recombination through H3K27 acetylation.

Chi W, Kang N, Sheng L, Liu S, Tao L, Cao X, Liu Y, Zhu C, Zhang Y, Wu B, Chen R, Cheng L, Wang J, Sun X, Liu X, Deng H, Yang J, Li Z, Liu W, Chen L
Nature communications 2024 Jan 2;15(1):163

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Zhao Q, Wang Q, Li B, Han S, Zhang Y, Wang Y, Lu R, Chen Q, Sun Z, Ding M, Liang Z, Gao Y
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Mitochondrial enzyme FAHD1 reduces ROS in osteosarcoma.

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Ferroptosis of CD163(+) tissue-infiltrating macrophages and CD10(+) PC(+) epithelial cells in lupus nephritis.

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Comprehensive isotopomer analysis of glutamate and aspartate in small tissue samples.

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Cell metabolism 2023 Oct 3;35(10):1830-1843.e5

Targeting BCAA metabolism to potentiate metformin's therapeutic efficacy in the treatment of diabetes in mice.

Zhao X, Zhang X, Pei J, Liu Y, Niu W, Sun H
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A bacteria-derived tetramerized protein ameliorates nonalcoholic steatohepatitis in mice via binding and relocating acetyl-coA carboxylase.

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Differential Inhibition of Anaplerotic Pyruvate Carboxylation and Glutaminolysis-Fueled Anabolism Underlies Distinct Toxicity of Selenium Agents in Human Lung Cancer.

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Journal of neurochemistry 2023 Feb;164(4):512-528

The adipose-derived stem cell peptide ADSCP2 alleviates hypertrophic scar fibrosis via binding with pyruvate carboxylase and remodeling the metabolic landscape.

Li J, Yin Y, Zou J, Zhang E, Li Q, Chen L, Li J
Acta physiologica (Oxford, England) 2023 Aug;238(4):e14010

Pyruvate anaplerosis is a targetable vulnerability in persistent leukaemic stem cells.

Rattigan KM, Brabcova Z, Sarnello D, Zarou MM, Roy K, Kwan R, de Beauchamp L, Dawson A, Ianniciello A, Khalaf A, Kalkman ER, Scott MT, Dunn K, Sumpton D, Michie AM, Copland M, Tardito S, Gottlieb E, Vignir Helgason G
Nature communications 2023 Aug 17;14(1):4634

LINC00092 Modulates Oxidative Stress and Glycolysis of Breast Cancer Cells via Pyruvate Carboxylase-Mediated AKT/mTOR Pathway.

Chen W, Liu Y, Kang S, Lv X, Fu W, Zhang J, Song C
Oxidative medicine and cellular longevity 2022;2022:5215748

Holocarboxylase synthetase knockout is embryonic lethal in mice.

Sadri M, Wang H, Kuroishi T, Li Y, Zempleni J
PloS one 2022;17(4):e0265539

Differential requirements for mitochondrial electron transport chain components in the adult murine liver.

Lesner NP, Wang X, Chen Z, Frank A, Menezes CJ, House S, Shelton SD, Lemoff A, McFadden DG, Wansapura J, DeBerardinis RJ, Mishra P
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Journal of immunology (Baltimore, Md. : 1950) 2022 Nov 1;209(9):1674-1690

The long noncoding RNA ADIPINT regulates human adipocyte metabolism via pyruvate carboxylase.

Kerr AG, Wang Z, Wang N, Kwok KHM, Jalkanen J, Ludzki A, Lecoutre S, Langin D, Bergo MO, Dahlman I, Mim C, Arner P, Gao H
Nature communications 2022 May 26;13(1):2958

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The protease SENP2 controls hepatic gluconeogenesis by regulating the SUMOylation of the fuel sensor AMPKα.

Dou X, Zhou WY, Ding M, Ma YJ, Yang QQ, Qian SW, Tang Y, Tang QQ, Liu Y
The Journal of biological chemistry 2022 Feb;298(2):101544

Requirement of hepatic pyruvate carboxylase during fasting, high fat, and ketogenic diet.

Selen ES, Rodriguez S, Cavagnini KS, Kim HB, Na CH, Wolfgang MJ
The Journal of biological chemistry 2022 Dec;298(12):102648

Enhancement of anaerobic glycolysis - a role of PGC-1α4 in resistance exercise.

Koh JH, Pataky MW, Dasari S, Klaus KA, Vuckovic I, Ruegsegger GN, Kumar AP, Robinson MM, Nair KS
Nature communications 2022 Apr 28;13(1):2324

A Short Isoform of Spermatogenic Enzyme GAPDHS Functions as a Metabolic Switch and Limits Metastasis in Melanoma.

Gill JG, Leef SN, Ramesh V, Martin-Sandoval MS, Rao AD, West L, Muh S, Gu W, Zhao Z, Hosler GA, Vandergriff TW, Durham AB, Mathews TP, Aurora AB
Cancer research 2022 Apr 1;82(7):1251-1266

PRDM16 Inhibits Cell Proliferation and Migration via Epithelial-to-Mesenchymal Transition by Directly Targeting Pyruvate Carboxylase in Papillary Thyroid Cancer.

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Wang H, Wu Y, Wang Z, Chen Y, Mo J, Guan W, Zhang Y, Yao H
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Zhang L, Liu T, Hu C, Zhang X, Zhang Q, Shi K
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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HepG2 cells were subjected to SDS PAGE followed by western blot with 16588-1-AP(PC antibody) at dilution of 1:1000
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of HepG2 cells, using PC antibody 16588-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human liver using 16588-1-AP(PC antibody) at dilution of 1:50 (under 10x lens)
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human liver using 16588-1-AP(PC antibody) at dilution of 1:50 (under 40x lens)
Sample type: tissue