16588-1-AP

antibody from Proteintech Group

Targeting: PC PCB

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

16588-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#16588-1-AP, RRID:AB_1851513

Product name

Pyruvate Carboxylase antibody

Antibody type

Polyclonal

Description

KD/KO validated Pyruvate Carboxylase antibody (Cat. #16588-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

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Complementary hepatic metabolomics and proteomics reveal the adaptive mechanisms of dairy cows to the transition period.

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Journal of dairy science 2023 Mar;106(3):2071-2088

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Characterization of huntingtin interactomes and their dynamic responses in living cells by proximity proteomics.

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Journal of neurochemistry 2023 Feb;164(4):512-528

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The adipose-derived stem cell peptide ADSCP2 alleviates hypertrophic scar fibrosis via binding with pyruvate carboxylase and remodeling the metabolic landscape.

Li J, Yin Y, Zou J, Zhang E, Li Q, Chen L, Li J
Acta physiologica (Oxford, England) 2023 Aug;238(4):e14010

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Pyruvate anaplerosis is a targetable vulnerability in persistent leukaemic stem cells.

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Nature communications 2023 Aug 17;14(1):4634

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LINC00092 Modulates Oxidative Stress and Glycolysis of Breast Cancer Cells via Pyruvate Carboxylase-Mediated AKT/mTOR Pathway.

Chen W, Liu Y, Kang S, Lv X, Fu W, Zhang J, Song C
Oxidative medicine and cellular longevity 2022;2022:5215748

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Holocarboxylase synthetase knockout is embryonic lethal in mice.

Sadri M, Wang H, Kuroishi T, Li Y, Zempleni J
PloS one 2022;17(4):e0265539

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Differential requirements for mitochondrial electron transport chain components in the adult murine liver.

Lesner NP, Wang X, Chen Z, Frank A, Menezes CJ, House S, Shelton SD, Lemoff A, McFadden DG, Wansapura J, DeBerardinis RJ, Mishra P
eLife 2022 Sep 26;11

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Sex differences in brain tumor glutamine metabolism reveal sex-specific vulnerabilities to treatment.

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Polarization and β-Glucan Reprogram Immunomodulatory Metabolism in Human Macrophages and Ex Vivo in Human Lung Cancer Tissues.

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The long noncoding RNA ADIPINT regulates human adipocyte metabolism via pyruvate carboxylase.

Kerr AG, Wang Z, Wang N, Kwok KHM, Jalkanen J, Ludzki A, Lecoutre S, Langin D, Bergo MO, Dahlman I, Mim C, Arner P, Gao H
Nature communications 2022 May 26;13(1):2958

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Identification of Pyruvate Carboxylase as the Cellular Target of Natural Bibenzyls with Potent Anticancer Activity against Hepatocellular Carcinoma via Metabolic Reprogramming.

Sheng Y, Chen Y, Zeng Z, Wu W, Wang J, Ma Y, Lin Y, Zhang J, Huang Y, Li W, Zhu Q, Wei X, Li S, Wisanwattana W, Li F, Liu W, Suksamrarn A, Zhang G, Jiao W, Wang F
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The Journal of biological chemistry 2022 Feb;298(2):101544

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The Journal of biological chemistry 2022 Dec;298(12):102648

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Enhancement of anaerobic glycolysis - a role of PGC-1α4 in resistance exercise.

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Nature communications 2022 Apr 28;13(1):2324

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A Short Isoform of Spermatogenic Enzyme GAPDHS Functions as a Metabolic Switch and Limits Metastasis in Melanoma.

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PRDM16 Inhibits Cell Proliferation and Migration via Epithelial-to-Mesenchymal Transition by Directly Targeting Pyruvate Carboxylase in Papillary Thyroid Cancer.

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SARS-CoV-2 infection rewires host cell metabolism and is potentially susceptible to mTORC1 inhibition.

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Cai D, Jia Y, Song H, Sui S, Lu J, Jiang Z, Zhao R
PloS one 2014;9(8):e105504

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Mesenchymal phenotype predisposes lung cancer cells to impaired proliferation and redox stress in response to glutaminase inhibition.

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Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 16588-1-AP(PC antibody) at dilution of 1:1000

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using PC antibody 16588-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human liver using 16588-1-AP(PC antibody) at dilution of 1:50 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human liver using 16588-1-AP(PC antibody) at dilution of 1:50 (under 40x lens)

Sample type

tissue