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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- R34431 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Pyruvate Carboxylase Antibody
- Antibody type
- Polyclonal
- Antigen
- Amino acids KFKEVKKAYVEANQ were used as the immunogen for this Pyruvate Carboxylase antibody.
- Description
- Antigen affinity purified antibody
- Reactivity
- Human, Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- Aliquot and store the Pyruvate Carboxylase antibody at -20°C.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot of HEK293 lysate overexpressing human Pyruvate Carboxylase probed with Pyruvate Carboxylase antibody (mock transfection in lane 1). Predicted molecular weight: ~130kDa, observed here at 140~150kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human liver tissue with Pyruvate Caboxylase antibody at 5ug/ml. HIER: steamed antigen retrieval with pH6 citrate buffer; AP-staining.
