AP02029SU-N

antibody from Acris Antibodies GmbH

Targeting: GPC4 K-glypican

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Recommended by provider)

Antibody Data

Product number

AP02029SU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AP02029SU-N, RRID:AB_1616776

Product name

anti Glypican-4 (GPC4) (509-520)

Antibody type

Polyclonal

Antigen

Synthetic Glypican-4 (aa 509-520) coupled to KLH.

Reactivity

Human, Mouse

Host

Rabbit

Vial size

0.1 ml

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western Blot analysis of Glypican-4 expression in tissues and cell lines. Proteoglycans from the cell or tissue extracts were separated by SDS-PAGE either prior to (N) or after (H) digestion with heparinase/heparitinase. Immunoblotting with Glypican-4 Antibody Cat.-No AP02029SU detects native Glypican-4 in the range of 90-200 kDa, the size of the core was Approx. 57 kDa. TF-1, Human haematopoietic-progenitor cell line; BM, bone marrow; Mr Molecular mass.  Siebertz B et al. (1999) Biochem. J. 344: 937-43.

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunohistochemistry of Glypica-4 staining in section of Human Kidney tissue. The section was incubated with Glypican-4 Antibody Cat.-No AP02029SU and detected using a peroxidase-conjugated secondary antibody according to standard procedures. In the cortex AP02029SU stains weakly the glomeruli, while the luminal surface of epithelial cells in the proximal tubules stained strongly. Original magnification: x1280. Siebertz B et al. (1999) Biochem. J. 344: 937-43.