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Western blot
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- AP02029SU-S - Provider product page
- Provider
- OriGene
- Product name
- Glypican 4 (GPC4) (509-520) rabbit polyclonal antibody, Serum
- Antibody type
- Polyclonal
- Description
- Glypican 4 (GPC4) (509-520) rabbit polyclonal antibody, Serum
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- GPC4
- Antibody clone number
- NULL
- Vial size
- 20 µl
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western Blot analysis of Glypican-4 expression in tissues and cell lines. Proteoglycans from the cell or tissue extracts were separated by SDS-PAGE either prior to (N) or after (H) digestion with heparinase/heparitinase. Immunoblotting with?Glypican-4 Antibody Cat.-No?AP02029SU detects native Glypican-4 in the range of 90-200 kDa, the size of the core was Approx. 57 kDa. TF-1, Human haematopoietic-progenitor cell line; BM, bone marrow; Mr Molecular mass.? Siebertz B et al. (1999) Biochem. J. 344: 937-43.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemistry of Glypica-4 staining in section of Human Kidney tissue. The section was incubated with?Glypican-4 Antibody Cat.-No?AP02029SU and detected using a peroxidase-conjugated secondary antibody according to standard procedures.?In the cortex AP02029SU stains weakly the glomeruli, while the luminal surface of epithelial cells in the proximal tubules stained strongly. Original magnification: x1280. Siebertz B et al. (1999) Biochem. J. 344: 937-43.
- Validation comment
- IHC
