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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- AM09357PU-S - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AM09357PU-S, RRID:AB_10650050
- Product name
- anti Glypican-4 (GPC4)
- Antibody type
- Monoclonal
- Antigen
- Recombinant human GPC4 (401-529 aa) purified from E. coli.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- AT4A3
- Vial size
- 50 µl
- Concentration
- 1.0 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis: Cell lysates of Mouse kidney lysates (35 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human GPC4 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Cell lysates of Mouse kidney lysates (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human GPC4 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunofluorescence: Human HeLa cells stained with Hoechst 3342 (Blue) for nucleus staining and monoclonal anti-human GPC4 antibody (1:500) with Texas Red (Red).
