21324-1-AP

antibody from Proteintech Group

Targeting: ZDHHC5 DHHC5, KIAA1748, ZNF375

Provider product page for 21324-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Flow cytometry

Antibody data

Antibody Data
Antigen structure
References [22]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]

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Product number: 21324-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#21324-1-AP, RRID:AB_10732816
Product name: ZDHHC5 antibody
Antibody type: Polyclonal
Description: KD/KO validated ZDHHC5 antibody (Cat. #21324-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IP, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

ZDHHC5 protein structure - 21324-1-AP shown in red.

Submitted references KIF13B controls ciliary protein content by promoting endocytic retrieval and suppressing release of large extracellular vesicles from cilia.

Rezi CK, Frei A, Campestre F, Boldt K, Mary B, Fixdahl AM, With Petersen AL, Sicot A, Berggreen CR, Laplace J, Johansen SL, Sørensen JKT, Chamlali M, Berchtold MW, Christensen ST, Anvarian Z, May-Simera HL, Pedersen LB
Current biology : CB 2025 Oct 6;35(19):4651-4667.e9

Palmitoylation enhances the stability of porcine epidemic diarrhea virus spike protein by antagonizing its degradation via chaperone-mediated autophagy to facilitate viral proliferation.

Qian Q, Zhao S-s, Yang L, Xing G, Chen Y, Liang C, Wang H, Li R, Qiao S, Wang A, Zhang G
Journal of virology 2025 Jun 17;99(6):e0034725

Neonatal sevoflurane exposures inhibits DHHC5-mediated palmitoylation of TfR1 in oligodendrocytes, leading to hypomyelination and neurological impairments.

Liu H, Su B, Zhang Z, Jia S, Wang J, Zhou F, Liu Y, Cao Q, Tang J, Ou Z, Zhang MM, Chen Y, Dong H, Zhong H
Journal of advanced research 2025 Dec;78:471-485

Targeting high mobility group protein B2 exerts antiproliferative effects in hypoxic pulmonary hypertension by modulating miR-21.

Wang P, Zhang X, Yao M, Li J, Wei X, Qiu Z, Chen L, Zhang L
Toxicology and applied pharmacology 2025 Apr;497:117265

Multifunctional Copper-Phenolic Nanopills Achieve Comprehensive Polyamines Depletion to Provoke Enhanced Pyroptosis and Cuproptosis for Cancer Immunotherapy.

Zhu G, Xie Y, Wang J, Wang M, Qian Y, Sun Q, Dai Y, Li C
Advanced materials (Deerfield Beach, Fla.) 2024 Nov;36(45):e2409066

ROS-dependent S-palmitoylation activates cleaved and intact gasdermin D.

Du G, Healy LB, David L, Walker C, El-Baba TJ, Lutomski CA, Goh B, Gu B, Pi X, Devant P, Fontana P, Dong Y, Ma X, Miao R, Balasubramanian A, Puthenveetil R, Banerjee A, Luo HR, Kagan JC, Oh SF, Robinson CV, Lieberman J, Wu H
Nature 2024 Jun;630(8016):437-446

Caveolin-2 palmitoylation turnover facilitates insulin receptor substrate-1-directed lipid metabolism by insulin receptor tyrosine kinase.

Choi M, Lee J, Jeong K, Pak Y
Biochimica et biophysica acta. Molecular basis of disease 2024 Jun;1870(5):167173

Atf3-mediated metabolic reprogramming in hepatic macrophage orchestrates metabolic dysfunction-associated steatohepatitis.

Hu S, Li R, Gong D, Hu P, Xu J, Ai Y, Zhao X, Hu C, Xu M, Liu C, Chen S, Fan J, Zhao Z, Zhang Z, Wu H, Xu Y
Science advances 2024 Jul 26;10(30):eado3141

ABHD7-mediated depalmitoylation of lamin A promotes myoblast differentiation.

Shen Y, Zheng LL, Fang CY, Xu YY, Wang C, Li JT, Lei MZ, Yin M, Lu HJ, Lei QY, Qu J
Cell reports 2024 Feb 27;43(2):113720

S-acylation of the Wnt receptor Frizzled-5 by zDHHC5 controls its cellular localization and synaptogenic activity in the rodent hippocampus.

Teo S, Bossio A, Stamatakou E, Pascual-Vargas P, Jones ME, Schuhmacher LN, Salinas PC
Developmental cell 2023 Oct 23;58(20):2063-2079.e9

ROS-dependent palmitoylation is an obligate licensing modification for GSDMD pore formation.

Du G, Healy LB, David L, Walker C, Fontana P, Dong Y, Devant P, Puthenveetil R, Ficarro SB, Banerjee A, Kagan JC, Lieberman J, Wu H
bioRxiv : the preprint server for biology 2023 Mar 7;

ZDHHC2-Mediated AGK Palmitoylation Activates AKT-mTOR Signaling to Reduce Sunitinib Sensitivity in Renal Cell Carcinoma.

Sun Y, Zhu L, Liu P, Zhang H, Guo F, Jin X
Cancer research 2023 Jun 15;83(12):2034-2051

Docosahexaenoic acid reverses PD-L1-mediated immune suppression by accelerating its ubiquitin-proteasome degradation.

Zhang H, Chen H, Yin S, Fan L, Jin C, Zhao C, Hu H
The Journal of nutritional biochemistry 2023 Feb;112:109186

Repositioning Lomitapide to block ZDHHC5-dependant palmitoylation on SSTR5 leads to anti-proliferation effect in preclinical pancreatic cancer models.

Wang Y, Zhang S, He H, Luo H, Xia Y, Jiang Y, Jiang J, Sun L
Cell death discovery 2023 Feb 11;9(1):60

ZDHHC5-mediated NLRP3 palmitoylation promotes NLRP3-NEK7 interaction and inflammasome activation.

Zheng S, Que X, Wang S, Zhou Q, Xing X, Chen L, Hou C, Ma J, An P, Peng Y, Yao Y, Song Q, Li J, Zhang P, Pei H
Molecular cell 2023 Dec 21;83(24):4570-4585.e7

Palmitoylation restricts SQSTM1/p62-mediated autophagic degradation of NOD2 to modulate inflammation.

Zhou L, He X, Wang L, Wei P, Cai Z, Zhang S, Jin S, Zeng H, Cui J
Cell death and differentiation 2022 Aug;29(8):1541-1551

AKAP150 and its Palmitoylation Contributed to Pain Hypersensitivity Via Facilitating Synaptic Incorporation of GluA1-Containing AMPA Receptor in Spinal Dorsal Horn.

Li Y, Bai X, Gao M, Chen H, Ma X, Zhang Y, Bai H, Liu Y, Hu X, Suo Z
Molecular neurobiology 2021 Dec;58(12):6505-6519

DHHC5 Mediates β-Adrenergic Signaling in Cardiomyocytes by Targeting Gα Proteins.

Chen JJ, Marsden AN, Scott CA, Akimzhanov AM, Boehning D
Biophysical journal 2020 Feb 25;118(4):826-835

miR-96-5p enhances cell proliferation and invasion via targeted regulation of ZDHHC5 in gastric cancer.

Wang B, Liu X, Meng X
Bioscience reports 2020 Apr 30;40(4)

MiR-96-5p inhibition induces cell apoptosis in gastric adenocarcinoma.

Zhou HY, Wu CQ, Bi EX
World journal of gastroenterology 2019 Dec 21;25(47):6823-6834

ZDHHC8 critically regulates seizure susceptibility in epilepsy.

Yang Q, Zheng F, Hu Y, Yang Y, Li Y, Chen G, Wang W, He M, Zhou R, Ma Y, Xu D, Tian X, Gao X, Wang Q, Wang X
Cell death & disease 2018 Jul 23;9(8):795

DHHC5-mediated palmitoylation of S1P receptor subtype 1 determines G-protein coupling.

Badawy SMM, Okada T, Kajimoto T, Ijuin T, Nakamura SI
Scientific reports 2017 Nov 29;7(1):16552

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21324-1-AP(ZDHHC5 antibody) at dilution of 1:500
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of Hela cells, using ZDHHC5 antibody 21324-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Sample type: cell line