Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- MAB4635 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rat Monoclonal IL-28A/IFN-lambda 2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse IL-28A/IFN-lambda 2 in direct ELISAs. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse IL-28B and no cross-reactivity with recombinant human (rh) IL-28A, rhIL-28B, or rhIL-29 is observed.
- Reactivity
- Mouse
- Host
- Rat
- Isotype
- IgG
- Vial size
- 500 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
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- Novus Biologicals (provider)
- Main image
- Experimental details
- IL-28A/IFN-lambda 2 Inhibition of EMCV-induced Cytopathy and Neutralization by Mouse IL-28A/IFN-lambda 2 Antibody. Recombinant Mouse IL-28A (Catalog # 4635-ML) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HepG2 human hepatocellular carcinoma cell line in a dose-dependent manner (orange line), as measured by Resazurin. Inhibition of EMCV activity elicited by Recombinant Mouse IL-28A (20 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL-28A Monoclonal Antibody (Catalog # MAB4635). The ND50 is typically 2-10 µg/mL.