Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF4635 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse IL-28A/IFN-lambda 2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse IL-28A/IFN-lambda 2 in direct ELISAs and Western blots. In Western blots, approximately 50% cross-reactivity with recombinant mouse IL-28B is observed and less than 5% cross-reactivity with recombinant human (rh) IL-28A and rhIL-28B is observed.
- Reactivity
- Mouse
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
NP_001019844
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IL-28A/IFN-lambda 2 Inhibition of EMCV-induced Cytopathy and Neutralization by Mouse IL-28A/IFN-lambda 2 Antibody. Recombinant Mouse IL-28A (Catalog # 4635-ML) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HepG2 human hepatocellular carcinoma cell line in a dose-dependent manner (orange line). Inhibition of EMCV activity elicited by Recombinant Mouse IL-28A (20 ng/mL) is neutralized (green line) by increasing concentrations of Sheep Anti-Mouse IL-28A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4635). The ND50 is typically 3-15 µg/mL.